Cntn6 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:61081 Lee S, et al., Expression and regulation of a gene encoding neural recognition molecule NB-3 of the contactin/F3 subgroup in mouse brain. Gene. 2000 Mar 21;245(2):253-66
Assay type:	Northern blot
MGI Accession ID:	MGI:4421765
Gene symbol:	Cntn6
Gene name:	contactin 6

Probe:	NB-3 cDNA1
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	The changes in expression were semi-quantitatively estimated by normalizing with the signal ratio relative to the GAPDH mRNA signals and are shown in Figure 2D.

Results

Image: 2B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
E17	E17.0 (a)	TS25: brain	Weak	10 µg; total RNA	ICR		Male
P1	P1	TS27: cerebellum	Weak	10 µg; total RNA	ICR		Male
P3	P3	TS27: cerebellum	Weak	10 µg; total RNA	ICR		Male
P7	P7	TS28: cerebellum	Present	10 µg; total RNA	ICR		Male
P10	P10	TS28: cerebellum	Present	10 µg; total RNA	ICR		Male
P14	P14	TS28: cerebellum	Present	10 µg; total RNA	ICR		Male
P21	P21	TS28: cerebellum	Present	10 µg; total RNA	ICR		Male
M3	P m 3	TS28: cerebellum	Strong (b)	10 µg; total RNA	ICR		Male
M6	P m 6	TS28: cerebellum	Strong (b)	10 µg; total RNA	ICR		Male

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression was low in early postnatal period and by 3-6 months it was approximately nine-fold greater than that on P 1.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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