Summary | Mutation origin | Mutation description | Expression | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm1(CAG-EGFP)Fsh
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Gordon Fishell
MGI ID:	MGI:4420759
Synonyms:	RCE:dual
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm1(CAG-EGFP)Fsh page

Germline Transmission:	Earliest citation of germline transmission: J:155164
Parent Cell Line:	W4 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Conditional ready, Reporter) Mutation:    Insertion   Mutation details: The R26R CAG-boosted EGFP (RCE) reporter targeting vector was designed with (from 5' to 3') the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector), a splice acceptor site, a loxP-flanked transcriptional STOP cassette (PGK-Neo-polyA and 3x-polyA), an FRT-flanked modified pBS302 STOP cassette, an enhanced green fluorescent protein (EGFP) sequence, and two AttP-polyA sequences. After removal of the flanked STOP cassettes via cre- and Flp-mediated recombination, the EGFP reporter is expressed in cells/tissues where the expression patterns of the individual promoters driving Cre recombinase and FLP recombinase overlap. (J:155164)

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	944 strains or lines available

Original:	J:155164 Sousa VH, et al., Characterization of Nkx6-2-derived neocortical interneuron lineages. Cereb Cortex. 2009 Jul;19 Suppl 1:i1-10
All:	20 reference(s)