Gene Expression Data
Assay Details
Assay
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Reference:
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J:155888
Rodriguez-Magadan H, et al., Sexually dimorphic gene expression of the Zimp7 and Zimp10 genes in embryonic gonads. Gene Expr Patterns. 2010 Jan;10(1):16-23
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4419098
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Gene symbol:
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Zmiz2
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Gene name:
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zinc finger, MIZ-type containing 2
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Antibody:
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Zimp7 antibody
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3A:
embryonic day 13.5
(more )
Specimen
3A:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Specimen
3B:
embryonic day 13.5
(more )
Specimen
3B:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Specimen
3C:
embryonic day 13.5
(more )
Note: Double labeled: green - Zmiz2; red - Pou5f1 (assay MGI:4420235).
Specimen
3C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Double labeled: green - Zmiz2; red - Pou5f1 (assay MGI:4420235).
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Specimen
4C:
postnatal day 30
(more )
Specimen
4C:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 30
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Sex:
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Male
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Present |
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4C
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Expression is in pachytene and leptotene/zygotene spermatocytes.
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TS28: spermatid |
Present |
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4C
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Expression is in elongated spermatids.
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Specimen
5D:
postnatal adult
(more )
Specimen
5D:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatogonium |
Present |
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5D
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Expression is in the cytoplasm and nucleus of spermatogonia.
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TS28: spermatocyte |
Present |
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5D
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Expression is in the nucleus of leptotene/zygotene spermatocytes.
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Specimen
5E:
postnatal adult
(more )
Specimen
5E:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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optical section
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Fixation:
|
2% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatogonium |
Present |
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5E
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Expression is in the cytoplasm and nucleus of spermatogonia.
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TS28: spermatocyte |
Present |
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5E
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Expression is in the nucleus of leptotene/zygotene spermatocytes.
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TS28: spermatid |
Strong |
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5E
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Expression is in the cytoplasm of round spermatids.
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Specimen
5 G,I:
postnatal adult
(more )
Note: Double labeled: green - Zmiz2; red - Sumo1 (assay MGI:4419097).
Specimen
5 G,I:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Double labeled: green - Zmiz2; red - Sumo1 (assay MGI:4419097).
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Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Strong |
Spotted |
5G
,
5I
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A punctate pattern in the nucleus and an intense signal in the XY body.
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Specimen
5 J,L:
postnatal adult
(more )
Note: Double labeled: green - Zmiz2; red - Sumo1 (assay MGI:4419097).
Specimen
5 J,L:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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optical section
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Fixation:
|
2% Paraformaldehyde
|
Note:
|
Double labeled: green - Zmiz2; red - Sumo1 (assay MGI:4419097).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Present |
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5L
,
5J
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A punctate pattern in the nucleus and an intense signal in the XY body and additional expression in the cytoplasm.
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