Gene Expression Data
Assay Details
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Assay
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Reference:
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J:155929
Soyer J, et al., Rfx6 is an Ngn3-dependent winged helix transcription factor required for pancreatic islet cell development. Development. 2010 Jan;137(2):203-12
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4418318
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Gene symbol:
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Rfx6
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Gene name:
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regulatory factor X, 6
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Antibody:
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anti-Rfx6
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Assay notes:
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The authors produced two antibodies but did not detail which was used in each experiment; it is possible MGI:4418272 was used to produce these data.
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Results
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Specimen
1D/E:
embryonic day 9.5
(more  )
Note: Double labeled: green - Rfx6; red - Pdx1 (assay MGI:4418319).
Specimen
1D/E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Pdx1 (assay MGI:4418319).
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Specimen
1G:
embryonic day 10.5
(more )
Note: Double labeled: green - Rfx6; red - Pdx1 (assay MGI:4418319).
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Pdx1 (assay MGI:4418319).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: pancreas primordium |
Present |
Regionally restricted |
1G
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Expression appears to be present in the differentiating endocrine (islet) cells of the pancreas.
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Specimen
1H:
embryonic day 10.5
(more )
Note: Double labeled: green - Rfx6; red - Ptf1a (assay MGI:4418320).
Specimen
1H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Ptf1a (assay MGI:4418320).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: pancreas primordium |
Present |
Regionally restricted |
1H
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Expression appears to be present in the differentiating endocrine (islet) cells of the pancreas.
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Specimen
2B/C:
embryonic day 10.5
(more )
Note: Double labeled: green - Rfx6; red - Neurog3 (assay MGI:4418321).
Specimen
2B/C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Neurog3 (assay MGI:4418321).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: pancreas primordium |
Present |
Regionally restricted |
2C
,
2B
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Expression appears to be present in the developing islet cells of the pancreas.
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Specimen
2E/F:
embryonic day 15.5
(more )
Note: Double labeled: green - Rfx6; red - Neurog3 (assay MGI:4418321).
Specimen
2E/F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Neurog3 (assay MGI:4418321).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
Regionally restricted |
2E
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2F
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Expression appears to be present in the developing islet cells of the pancreas.
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Specimen
5A:
embryonic day 10.5
(more )
Note: Double labeled: green - Rfx6; red - Gcg (assay MGI:4418322).
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Gcg (assay MGI:4418322).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: foregut-midgut junction |
Present |
Regionally restricted |
5A
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Expression is present in the developing alpha cells of the pancreas.
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Specimen
5B:
embryonic day 15.5
(more )
Note: Double labeled: green - Rfx6; red - Gcg (assay MGI:4418322).
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Gcg (assay MGI:4418322).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
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5B
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Expression is present in the developing alpha cells of the pancreas.
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Specimen
5C:
embryonic day 15.5
(more )
Note: Double labeled: green - Rfx6; red - Ins1 (assay MGI:4418323) and Ins2 (assay MGI:4418338).
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Ins1 (assay MGI:4418323) and Ins2 (assay MGI:4418338).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
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5C
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Expression is present in the developing beta cells of the pancreas.
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Specimen
5D:
postnatal adult
(more )
Note: Double labeled: green - Rfx6; red - Gcg (assay MGI:4418322).
Specimen
5D:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Gcg (assay MGI:4418322).
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Specimen
5E:
postnatal adult
(more )
Note: Double labeled: green - Rfx6; red - Ins1 (assay MGI:4418323) and Ins2 (assay MGI:4418338).
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Ins1 (assay MGI:4418323) and Ins2 (assay MGI:4418338).
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Specimen
5F:
postnatal adult
(more )
Note: Double labeled: green - Rfx6; red - Sst (assay MGI:4418324).
Specimen
5F:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Sst (assay MGI:4418324).
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Specimen
5G:
postnatal adult
(more )
Note: Double labeled: green - Rfx6; red - Ppy (assay MGI:4418325).
Specimen
5G:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Ppy (assay MGI:4418325).
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Specimen
S2 A:
embryonic day 12.5
(more )
Note: Double labeled: green - Rfx6; red - Mki67 (assay MGI:4418326).
Specimen
S2 A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Mki67 (assay MGI:4418326).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pancreas |
Present |
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S2_A
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Expression is primarily present in non-dividing cells; only rarely is it present in dividing cells.
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Specimen
S2 B:
embryonic day 13.5
(more )
Note: Double labeled: green - Rfx6; red - Mki67 (assay MGI:4418326).
Specimen
S2 B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Mki67 (assay MGI:4418326).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pancreas |
Present |
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S2_B
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Expression is primarily present in non-dividing cells; only rarely is it present in dividing cells.
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Specimen
S2 C:
embryonic day 15.5
(more )
Note: Double labeled: green - Rfx6; red - Mki67 (assay MGI:4418326).
Specimen
S2 C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Mki67 (assay MGI:4418326).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
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S2_C
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Expression is primarily present in non-dividing cells; only rarely is it present in dividing cells.
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Specimen
S2 D:
embryonic day 18.5
(more )
Note: Double labeled: green - Rfx6; red - Mki67 (assay MGI:4418326).
Specimen
S2 D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rfx6; red - Mki67 (assay MGI:4418326).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: pancreas |
Present |
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S2_D
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Expression is primarily present in non-dividing cells; only rarely is it present in dividing cells.
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