Gene Expression Data
Assay Details
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Assay
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Reference:
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J:155929
Soyer J, et al., Rfx6 is an Ngn3-dependent winged helix transcription factor required for pancreatic islet cell development. Development. 2010 Jan;137(2):203-12
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4418300
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Gene symbol:
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Rfx6
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Gene name:
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regulatory factor X, 6
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Probe:
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Rfx6 cDNA
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Probe preparation:
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Antisense RNA
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Results
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Specimen
1A:
embryonic day 9.0
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
1B:
embryonic day 9.0
(more )
Note: This is a section from the embryo pictured in Fig. 1A.
Specimen
1B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This is a section from the embryo pictured in Fig. 1A.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: gut epithelium |
Present |
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1B
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There is broad expression in the gut endoderm.
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Specimen
1F:
embryonic day 10.5
(more )
Note: Double labeled: blue - Rfx6; brown - Pdx1 (assay MGI:4418319).
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Rfx6; brown - Pdx1 (assay MGI:4418319).
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Specimen
2G:
embryonic day 15.5
(more )
Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
Regionally restricted |
2G
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Expression appears to be present in the developing islet cells of the pancreas.
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Neurog3tm1.1Ggr/Neurog3tm1.1Ggr
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Neurog3tm1.1Ggr/Neurog3tm1.1Ggr
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: foregut-midgut junction |
Weak |
Regionally restricted |
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Expression is almost completely ablated in the mutant.
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Specimen
3A:
embryonic day 15.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3C:
embryonic day 15.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3E:
embryonic day 15.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4B:
embryonic day 14.5
(more )
Specimen
4B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4D:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Neurod1tm1Mjts/Neurod1tm1Mjts
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: pancreas |
Present |
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4D
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Expression in the mutant is the same as in wild type.
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Specimen
4F:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Arxtm1Pgr/Arxtm1Pgr
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: pancreas |
Present |
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4F
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Expression in the mutant is the same as in wild type.
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Specimen
4H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Pax4tm1Pgr/Pax4tm1Pgr
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: pancreas |
Present |
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4H
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Expression in the mutant is the same as in wild type.
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Analysis Tools