Probe:	Znt3 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Present	Diffuse	1A
TS26: olfactory nerve	Present	Diffuse	1A

Structure	Level	Pattern	Image	Note
TS26: caudate-putamen	Present		1B
TS26: cortical plate	Present		1B
TS26: olfactory cortex subventricular zone	Present		1B
TS26: piriform cortex	Present		1B

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Weak		1C
TS26: thalamus	Weak		1C
TS26: cerebral cortex	Present	Regionally restricted	1C	Expression is observed in the retrosplenial area.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		1E
TS26: cerebral cortex ventricular zone	Present		1E
TS26: cortical plate	Present		1E

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present		2A
TS27: accessory olfactory bulb	Strong		2A

Structure	Level	Pattern	Image	Note
TS28: accessory olfactory bulb	Strong		2B
TS28: main olfactory bulb mitral cell layer	Present		2B

Structure	Level	Pattern	Image	Note
TS27: anterior commissure	Present	Diffuse	2C
TS27: corpus callosum	Present	Diffuse	2C
TS27: cingulate cortex	Weak	Regionally restricted	2C	Expression is in the upper layers of the cingular cortex.
TS27: brain ependyma	Weak		2C
TS27: brain subventricular zone	Weak		2C
TS27: corpus striatum	Weak		2C
TS27: telencephalon septum	Weak		2C

Structure	Level	Pattern	Image	Note
TS27: cingulate cortex	Present		2D
TS27: anterior commissure	Present	Diffuse	2D
TS27: corpus callosum	Present	Diffuse	2D

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Present		2E
TS28: brain ependyma	Weak		2E
TS28: brain subventricular zone	Weak		2E
TS28: caudate-putamen	Weak		2E
TS28: neocortex	Strong	Regionally restricted	2E	Expression is mainly in the upper layers of the different cortical areas (motor, somatosensorial, cingular).
TS28: cingulate cortex	Strong	Regionally restricted	2E	Expression is in the upper layers of the cingular cortex.
TS28: telencephalon septum	Absent		2E

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Present		2F
TS28: brain subventricular zone	Weak		2F

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Present		2G
TS28: brain subventricular zone	Weak		2G

Structure	Level	Pattern	Image	Note
TS27: cerebral hemisphere white matter	Present	Regionally restricted	3A	Expression is observed in the retrosplenial area.
TS27: dentate gyrus	Absent		3A
TS27: hippocampus	Absent		3A

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present		3B
TS28: hippocampus CA1	Present		3B
TS28: hippocampus CA3	Present		3B
TS28: piriform cortex	Present		3B

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present		3C

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present		3D
TS28: dentate gyrus hilus	Weak		3D

Structure	Level	Pattern	Image	Note
TS27: amygdala	Present		4A
TS27: entorhinal cortex	Present		4A
TS27: piriform cortex	Present		4A

Structure	Level	Pattern	Image	Note
TS28: entorhinal cortex	Present	Graded	4B	An increase of label was detected in lower layers of the entorhinal and piriform cortices.
TS28: piriform cortex	Present	Graded	4B	An increase of label was detected in lower layers of the entorhinal and piriform cortices.
TS28: amygdala	Present		4B

Structure	Level	Pattern	Image	Note
TS28: amygdala	Present	Regionally restricted	4C	Expression is in the lateral amygdaloid dorsolateral nucleus.
TS28: basolateral amygdaloid nucleus	Weak		4C
TS28: nucleus of lateral olfactory tract	Weak		4C
TS28: paraventricular thalamic nucleus	Present		4C
TS28: posterior thalamic nucleus	Present		4C
TS28: anterior cortical amygdaloid nucleus	Absent		4C

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Present		5A

Structure	Level	Pattern	Image	Note
TS27: medulla oblongata	Present	Diffuse	5B

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		5C
TS28: cerebellum external granule cell layer	Weak		5C

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		5D
TS28: cerebellum external granule cell layer	Weak		5D

Structure	Level	Pattern	Image	Note
TS28: accessory olfactory bulb	Present		6A
TS28: main olfactory bulb	Weak		6A

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Strong		6B	High expression in the upper layers of the cingular and the retrosplenial cortices.
TS28: cortical layer II	Present		6B
TS28: cortical layer III	Present		6B
TS28: cortical layer V	Weak	Single cells	6B
TS28: brain subventricular zone	Absent		6B
TS28: caudate-putamen	Absent		6B
TS28: corpus callosum	Absent		6B

Structure	Level	Pattern	Image	Note
TS28: hippocampus granule cell layer	Strong		6D
TS28: hippocampus pyramidal cell layer	Strong		6D
TS28: cingulate cortex	Strong		6D	High expression in the upper layers of the cingular and the retrosplenial cortices.
TS28: cortical layer V	Present		6D
TS28: hippocampus CA1	Present		6D
TS28: hippocampus CA3	Present		6D
TS28: paraventricular thalamic nucleus	Present		6D
TS28: thalamus	Present	Regionally restricted	6D	Expression is in the intermediodorsal thalamic nuclei.
TS28: cortical layer IV	Absent		6D
TS28: hippocampus CA2	Absent		6D

Structure	Level	Pattern	Image	Note
TS28: nucleus of lateral olfactory tract	Moderate		6E
TS28: piriform cortex	Strong		6E

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Strong		6F
TS28: basolateral amygdaloid nucleus	Weak		6F
TS28: amygdala	Present		6F	Expression is in the lateral amygdaloid dorsolateral nucleus.

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Present		6G

Structure	Level	Pattern	Image	Note
TS28: cerebellar cortex	Absent		6H	Expression is absent in all cerebellar layers.

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Strong		6I
TS28: dentate gyrus granule cell layer	Strong		6I
TS28: hippocampus CA3	Strong		6I
TS28: hippocampus granule cell layer	Strong		6I
TS28: hippocampus pyramidal cell layer	Strong		6I
TS28: cortical layer V	Present		6I
TS28: cortical layer IV	Absent		6I
TS28: hippocampus CA2	Absent		6I

Structure	Level	Pattern	Image	Note
TS28: deep cerebellar nucleus	Present	Regionally restricted		Expression is in the cerebellar nuclei, i.e., the posterior pons, the trapezoid body, and the superior cerebellar peduncle.