Probe:	5' probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	It is possible that another probe (3' probe MGI:4412255) was used in this assay.

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: neural crest	Present	Spotted	2A	Spotted specific staining consistent with expression in neural crest cells.

Structure	Level	Pattern	Image	Note
TS15: neural crest	Present	Spotted	2B	Spotted specific staining consistent with expression in neural crest cells.
TS15: migrating neural crest	Present	Single cells	2B	Expression is in migratory streams towards the dorsal portion of the 2nd, 3rd and 4th branchial arches and surrounding the otic vesicle.

Structure	Level	Pattern	Image	Note
TS16: migrating neural crest	Strong	Regionally restricted	2C	Expression is around the otic vesicle and in migratory streams towards the dorsal portion of the 2nd, 3rd and 4th branchial arches.
TS16: 1st branchial arch	Present	Regionally restricted	2C	Expression is ventral.
TS16: 2nd branchial arch	Present	Regionally restricted	2C	Expression is ventral.
TS16: 3rd branchial arch	Present	Regionally restricted	2C	Expression is ventral.
TS16: 4th branchial arch	Present	Regionally restricted	2C	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Present	Regionally restricted	2D	Expression is ventral.
TS16: 2nd branchial arch	Present	Regionally restricted	2D	Expression is ventral.
TS16: 3rd branchial arch	Present	Regionally restricted	2D	Expression is ventral.
TS16: 4th branchial arch	Present	Regionally restricted	2D	Expression is ventral.
TS16: nasal pit	Present	Scattered	2D	Expression is in few scattered cells.
TS16: trigeminal ganglion	Present	Scattered	2D	Expression is in few scattered cells.
TS16: eye	Present	Regionally restricted	2D	Expression is over the eye anlagen directly adjacent to the optic vesicle.
TS16: heart	Present	Regionally restricted	2D	Expression is in a stream of single stained cells superficially on the right side of the heart overlying the inflow tract/sinus venosus.
TS16: migrating neural crest	Present	Regionally restricted	2D	Expression is lateral to the aortic vessels in the lower cervical region where the sympathetic chain starts to condense from migrating neural crest cells.
TS16: peripheral nervous system	Present	Regionally restricted	2D	Expression is lateral to the aortic vessels in the lower cervical region where the sympathetic chain starts to condense from migrating neural crest cells.
TS16: septum transversum	Present		2D

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Present	Regionally restricted	2E	Expression is ventral.
TS16: 2nd branchial arch	Present	Regionally restricted	2E	Expression is ventral.
TS16: 3rd branchial arch	Present	Regionally restricted	2E	Expression is ventral.
TS16: 4th branchial arch	Present	Regionally restricted	2E	Expression is ventral.
TS16: heart	Present	Regionally restricted	2E	Expression is in a stream of single stained cells superficially on the right side of the heart overlying the inflow tract/sinus venosus.
TS16: migrating neural crest	Present	Regionally restricted	2E	Expression is lateral to the aortic vessels in the lower cervical region where the sympathetic chain starts to condense from migrating neural crest cells.
TS16: peripheral nervous system	Present	Regionally restricted	2E	Expression is lateral to the aortic vessels in the lower cervical region where the sympathetic chain starts to condense from migrating neural crest cells.
TS16: septum transversum	Present		2E

Structure	Level	Pattern	Image	Note
TS22: eye	Present		3a , 3A
TS22: pre-cartilage condensation	Present		3A	Expression is in proliferating mesenchyme-derived cells, including precartilage, cartilage, perichondrium, chondrifying bones, as well as in muscle structures.
TS22: skeletal muscle	Present		3A	Expression is in proliferating mesenchyme-derived cells, including precartilage, cartilage, perichondrium, chondrifying bones, as well as in muscle structures.
TS22: skeleton	Present		3A	Expression is in proliferating mesenchyme-derived cells, including precartilage, cartilage, perichondrium, chondrifying bones, as well as in muscle structures.
TS22: basioccipital cartilage condensation	Strong		3A
TS22: basisphenoid cartilage condensation	Strong		3A
TS22: rib cartilage condensation	Strong		3A	Expression is in all chondrified parts of the costal elements from the first rib on, and the costal cartilage in the ventral part of the ribcage.
TS22: femur cartilage condensation	Present		3A
TS22: fibula cartilage condensation	Present		3A
TS22: forelimb phalanx cartilage condensation	Present		3A
TS22: hindlimb phalanx cartilage condensation	Present		3A
TS22: humerus cartilage condensation	Present		3A
TS22: metacarpus cartilage condensation	Present		3A
TS22: metatarsus cartilage condensation	Present		3A
TS22: pelvic girdle skeleton	Present		3A
TS22: radius cartilage condensation	Present		3A
TS22: scapula cartilage condensation	Present		3A
TS22: tibia cartilage condensation	Present		3A
TS22: ulna cartilage condensation	Present		3A
TS22: tail mesenchyme	Weak	Regionally restricted	3A	Expression is in muscle tissues in the most caudal parts of the tail region.
TS22: embryo mesenchyme	Present	Regionally restricted	3A	Expression is in subepithelial mesenchyme.
TS22: skin	Present		3A

Structure	Level	Pattern	Image	Note
TS22: pre-cartilage condensation	Present		3B	Expression is in proliferating mesenchyme-derived cells, including precartilage, cartilage, perichondrium, chondrifying bones, as well as in muscle structures.
TS22: skeletal muscle	Present		3B	Expression is in proliferating mesenchyme-derived cells, including precartilage, cartilage, perichondrium, chondrifying bones, as well as in muscle structures.
TS22: skeleton	Present		3B	Expression is in proliferating mesenchyme-derived cells, including precartilage, cartilage, perichondrium, chondrifying bones, as well as in muscle structures.
TS22: basioccipital cartilage condensation	Strong		3B
TS22: basisphenoid cartilage condensation	Strong		3B
TS22: Meckel's cartilage	Present		3B
TS22: femur cartilage condensation	Present		3B
TS22: fibula cartilage condensation	Present		3B
TS22: forelimb phalanx cartilage condensation	Present		3B
TS22: hindlimb phalanx cartilage condensation	Present		3B
TS22: humerus cartilage condensation	Present		3B
TS22: mandible	Present		3B
TS22: metacarpus cartilage condensation	Present		3B
TS22: metatarsus cartilage condensation	Present		3B
TS22: pelvic girdle skeleton	Present		3B
TS22: radius cartilage condensation	Present		3B
TS22: scapula cartilage condensation	Present		3B
TS22: skin	Present		3B
TS22: tibia cartilage condensation	Present		3B
TS22: ulna cartilage condensation	Present		3B
TS22: vertebral cartilage condensation	Present		3B
TS22: rib cartilage condensation	Strong		3B , 3b , 3b'	Expression is in all chondrified parts of the costal elements from the first rib on, and the costal cartilage in the ventral part of the ribcage.
TS22: tail mesenchyme	Weak	Regionally restricted	3B	Expression is in muscle tissues in the most caudal parts of the tail region.
TS22: nose	Present	Regionally restricted	3B	Expression is in the cartilage primordium of the turbinate bones. Expression is strong in the most distal tip.
TS22: olfactory epithelium	Present	Regionally restricted	3B
TS22: vomeronasal organ	Strong		3B
TS22: embryo mesenchyme	Present	Regionally restricted	3B	Expression is in subepithelial mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: Meckel's cartilage	Present		3C , 3c
TS22: mandible	Present		3C , 3c
TS22: vertebral cartilage condensation	Present		3C , 3c
TS22: pre-cartilage condensation	Present		3C	Expression is in proliferating mesenchyme-derived cells, including precartilage, cartilage, perichondrium, chondrifying bones, as well as in muscle structures.
TS22: skeletal muscle	Present		3C	Expression is in proliferating mesenchyme-derived cells, including precartilage, cartilage, perichondrium, chondrifying bones, as well as in muscle structures.
TS22: skeleton	Present		3C	Expression is in proliferating mesenchyme-derived cells, including precartilage, cartilage, perichondrium, chondrifying bones, as well as in muscle structures.
TS22: basioccipital cartilage condensation	Strong		3C
TS22: basisphenoid cartilage condensation	Strong		3C
TS22: rib cartilage condensation	Strong		3C	Expression is in all chondrified parts of the costal elements from the first rib on, and the costal cartilage in the ventral part of the ribcage.
TS22: femur cartilage condensation	Present		3C
TS22: fibula cartilage condensation	Present		3C
TS22: forelimb phalanx cartilage condensation	Present		3C
TS22: hindlimb phalanx cartilage condensation	Present		3C
TS22: humerus cartilage condensation	Present		3C
TS22: metacarpus cartilage condensation	Present		3C
TS22: metatarsus cartilage condensation	Present		3C
TS22: pelvic girdle skeleton	Present		3C
TS22: radius cartilage condensation	Present		3C
TS22: scapula cartilage condensation	Present		3C
TS22: skin	Present		3C
TS22: tibia cartilage condensation	Present		3C
TS22: ulna cartilage condensation	Present		3C
TS22: tail mesenchyme	Weak	Regionally restricted	3C	Expression is in muscle tissues in the most caudal parts of the tail region.
TS22: embryo mesenchyme	Present	Regionally restricted	3C	Expression is in subepithelial mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: cornea epithelium	Present		3d , 3D , 3d'
TS22: eyelid epithelium	Present		3d , 3D , 3d'
TS22: lens anterior epithelium	Present		3d , 3D , 3d'
TS22: neural retinal epithelium	Present	Graded	3d' , 3D , 3d	There is an asymmetric distribution with more expression at the basal side of the epithelium.

Structure	Level	Pattern	Image	Note
TS22: cornea epithelium	Present		3E , 3E'
TS22: eyelid epithelium	Present		3E , 3E'
TS22: lens anterior epithelium	Present		3E , 3E'
TS22: neural retinal epithelium	Present	Graded	3E' , 3E	There is an asymmetric distribution with more expression at the basal side of the epithelium.

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present		3F , 3F'

Structure	Level	Pattern	Image	Note
TS22: tibia cartilage condensation	Present		3G