Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:153971
Giovannone B, et al., GIGYF2 gene disruption in mice results in neurodegeneration and altered insulin-like growth factor signaling. Hum Mol Genet. 2009 Dec 1;18(23):4629-39
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:4410285
|
|
Gene symbol:
|
Gigyf2
|
|
Gene name:
|
GRB10 interacting GYF protein 2
|
|
Reporter:
|
lacZ
|
|
|
Direct Detection
|
|
|
Results
|
Specimen
+/-:
(close  )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Gigyf2Gt(XH076)Byg/Gigyf2+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
-/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Gigyf2Gt(XH076)Byg/Gigyf2Gt(XH076)Byg
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: embryo |
Present |
|
3A E11.5 -/-
|
There is broad expression in embryonic tissues. Expression is more intense in homozygotes compared to heterozygotes.
|
| TS19: central nervous system |
Strong |
|
3A E11.5 -/-
|
|
Specimen
+/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Gigyf2Gt(XH076)Byg/Gigyf2+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
-/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Gigyf2Gt(XH076)Byg/Gigyf2Gt(XH076)Byg
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: embryo |
Present |
|
3A E14.5 -/-
|
There is broad expression in embryonic tissues. Expression is more intense in homozygotes compared to heterozygotes.
|
| TS22: central nervous system |
Strong |
|
3A E14.5 -/-
|
|
Specimen
3Ba:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Gigyf2Gt(XH076)Byg/Gigyf2Gt(XH076)Byg
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3Be:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Gigyf2Gt(XH076)Byg/Gigyf2Gt(XH076)Byg
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3C neurons:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Gigyf2Gt(XH076)Byg/Gigyf2Gt(XH076)Byg
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain |
Strong |
|
3C top
|
High intensity neuronal staining was evident in multiple brain regions.
|
Specimen
3C glia:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Gigyf2Gt(XH076)Byg/Gigyf2Gt(XH076)Byg
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain |
Present |
|
3C bottom
|
High intensity glial staining was evident in multiple brain regions.
|
|
Analysis Tools
)