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Gene Expression Data
Assay Details
Assay
Reference: J:146657 Witte F, et al., Comprehensive expression analysis of all Wnt genes and their major secreted antagonists during mouse limb development and cartilage differentiation. Gene Expr Patterns. 2009 Apr;9(4):215-23
Assay type: RNA in situ
MGI Accession ID: MGI:4397451
Gene symbol: Wif1
Gene name: Wnt inhibitory factor 1
Probe: Wif1 cDNA4
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 3A WM: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: forelimb bud mesenchyme Present Regionally restricted 3A Expression is in the anterior and posterior forelimb.
TS15: hindlimb ridge mesenchyme Present 3A

Specimen 3a sxn: embryonic day 9.5 (more )
Note: Vibratome section
Structure Level Pattern Image Note
TS15: hindlimb ridge mesenchyme Present Regionally restricted 3a Expression is distal.

Specimen 3B WM: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present Regionally restricted 3B The expression domain is across the anterior-posterior axis and is absent at the most proximal and distal mesenchyme.
TS17: otocyst Present 3B
TS17: somite Present 3B

Specimen 3b sxn: embryonic day 10.5 (more )
Note: Vibratome section
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3b The expression domain is across the anterior-posterior axis and is absent at the most proximal and distal mesenchyme.

Specimen 3b': embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: hindlimb bud mesenchyme Present Regionally restricted 3b' Expression is in the anterior and posterior mesenchyme as well as in the distal mesenchyme.

Specimen 3C WM: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: future arm mesenchyme Present Regionally restricted 3C Expression is in proximal mesenchyme and in anterior and posterior domains, stronger staining is coincident with the condensations of humerus, radius and ulna.
TS19: somite Present 3C
TS19: inner ear Present Regionally restricted 3C Expression is in the otic vesicle.

Specimen 3c sxn: embryonic day 11.5 (more )
Note: Vibratome section
Structure Level Pattern Image Note
TS19: future arm mesenchyme Present Regionally restricted 3c Expression is in proximal mesenchyme and in anterior and posterior domains, stronger staining is coincident with the condensations of humerus, radius and ulna.

Specimen 3c': embryonic day 11.5 (more )
Note: Vibratome section
Structure Level Pattern Image Note
TS19: future arm mesenchyme Present Regionally restricted 3c' Expression is in proximal mesenchyme and in anterior and posterior domains, stronger staining is coincident with the condensations of humerus, radius and ulna.
TS19: handplate mesenchyme Present Regionally restricted 3c' Expression is in anterior and posterior mesenchyme

Specimen 3c'': embryonic day 11.5 (more )
Note: Vibratome section
Structure Level Pattern Image Note
TS19: footplate mesenchyme Strong Regionally restricted 3c'' Strong staining in the medial region of the limb bud was seen, together with signals in the proximalmost anterior and posterior mesenchyme and in the distal anterior subridge mesenchyme.

Specimen 3D WM: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Strong Regionally restricted 3D Expression is in anterior and posterior proximal mesenchyme in proximal regions and in superficial mesenchyme.
TS20: hand mesenchyme Present Regionally restricted 3D Expression is in distal mesenchyme.

Specimen 3d sxn: embryonic day 12.5 (more )
Note: Vibratome section
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present Regionally restricted 3d Expression is in anterior and posterior proximal mesenchyme in proximal regions and in superficial mesenchyme.
TS20: hand mesenchyme Present Regionally restricted 3d Expression is in distal mesenchyme.

Specimen 3E WM: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: hand mesenchyme Present Regionally restricted 3E Expression is in distal mesenchyme and in dorsal tendons.

Specimen 3e sxn: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: hand Present Regionally restricted 3e Expression is in dorsal tendons.

Specimen 3F,f: embryonic day 13.5 (more )
Note: (f) is a higher magnification of the boxed area in (F).
Structure Level Pattern Image Note
TS21: hand mesenchyme Present Regionally restricted 3f , 3F Expression is distal.
TS21: forelimb interdigital region mesenchyme Strong 3F , 3f
TS21: hand pre-cartilage condensation Present 3f , 3F

Specimen 3G,g,g': embryonic day 15.5 (more )
Note: (g,g') are higher magnifications of the boxed areas in (G).
Structure Level Pattern Image Note
TS23: radius Present Regionally restricted 3g' , 3G , 3g Expression is in the distal periarticular perichondrium, absent in the growth plates.
TS23: ulna Present Regionally restricted 3g' , 3G , 3g Expression is in the distal periarticular perichondrium, absent in the growth plates.
TS23: forelimb mesenchyme Present Regionally restricted 3g' , 3g , 3G Expression is in tendons.
TS23: forelimb musculature Present 3g , 3g' , 3G
TS23: forelimb dermis Weak Patchy 3g , 3G
TS23: carpus Present Regionally restricted 3g , 3G Expression is in the center.
TS23: metacarpus Present Regionally restricted 3g , 3G Expression is in the center.

Specimen 3H: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: elbow Present Regionally restricted 3H Expression is in periarticular perichondrium.
TS23: forelimb mesenchyme Present Regionally restricted 3H Expression is in tendons.
TS23: forelimb musculature Present 3H

Specimen 3I: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: forelimb digit Present Regionally restricted 3I Expression is in periarticular perichondrium.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory