Antibody:	Anti-Foxn1
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Present	Regionally restricted	1b	Expression in nuclei of thymic cells.

Structure	Level	Pattern	Image	Note
TS19: foregut gland	Absent		2a	No expression in thymus anlage.

Structure	Level	Pattern	Image	Note
TS20: thymus primordium	Present	Regionally restricted	2b	Expression in many keratin positive cells in the thymus anlage.

Structure	Level	Pattern	Image	Note
TS20: thymus primordium	Present	Regionally restricted	2c	Expression in almost all epithelial cells in the thymus anlage.

Structure	Level	Pattern	Image	Note
TS24: thymus	Present	Regionally restricted	2d	Expression in a majority of keratin-positive cells, but absent in a few epithelial cells distributed among them.

Structure	Level	Pattern	Image	Note
TS27: thymus epithelium	Present	Regionally restricted	2e	Authors report that more epithelial cells have lost expression.

Structure	Level	Pattern	Image	Note
TS28: thymus epithelium	Present	Regionally restricted	2f	Expression in only a small subset of keratin-positive cells.