Antibody:	Anti-Calpastatin (H-300)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Slides were dried briefly and fixed in 10% formaldehyde including 1% t-octylphenoxypoly-ethoxyethanol.

Structure	Level	Pattern	Image	Note
TS22: molar enamel organ	Weak	Diffuse	2k

Structure	Level	Pattern	Image	Note
TS24: molar enamel organ	Weak	Diffuse	2l

Structure	Level	Pattern	Image	Note
TS26: molar	Present	Regionally restricted	2m	Expression was detected between enamel matrix and enamel organ.

Structure	Level	Pattern	Image	Note
TS27: enamel organ	Present	Regionally restricted	2n	Expression was detected in the extracellular enamel matrix.

Structure	Level	Pattern	Image	Note
TS28: molar	Present	Regionally restricted	2o	Expression was detected in odontoblasts.

Structure	Level	Pattern	Image	Note
TS26: skin	Present		3Ac

Structure	Level	Pattern	Image	Note
TS26: molar	Present	Regionally restricted	3Bc	Expression was detected between enamel matrix and enamel organ.

Structure	Level	Pattern	Image	Note
TS27: molar	Present	Regionally restricted	3Bf	Expression was detected in the extracellular enamel matrix, and the staining vanished at the point of the initial tooth mineralization.

Structure	Level	Pattern	Image	Note
TS28: molar	Present	Regionally restricted	3Cc	Expression was detected in odontoblasts.

Structure	Level	Pattern	Image	Note
TS28: molar	Present	Regionally restricted	3Cc'	Expression was detected in odontoblasts.

Structure	Level	Pattern	Image	Note
TS22: molar dental lamina	Weak
TS22: oral epithelium	Weak
TS22: molar epithelium	Weak	Regionally restricted		Expression was weak in outer enamel epithelium.

Structure	Level	Pattern	Image	Note
TS24: molar dental lamina	Weak
TS24: oral epithelium	Weak
TS24: molar epithelium	Weak	Regionally restricted		Expression was weak in the outer enamel epithelium.

Structure	Level	Pattern	Image	Note
TS26: molar dental lamina	Moderate
TS26: oral epithelium	Moderate
TS26: molar epithelium	Weak	Regionally restricted		Expression was weak in outer enamel epithelium, inner enamel epithelium, and odontoblasts.
TS26: stratum intermedium	Weak
TS26: molar	Moderate	Regionally restricted		Expression was moderate in extracellular (enamel, dentin) space.

Structure	Level	Pattern	Image	Note
TS27: dental lamina	Moderate
TS27: oral epithelium	Moderate
TS27: stratum intermedium	Moderate
TS27: molar	Present	Regionally restricted		Expression was strong in extracellular (enamel, dentin) space, moderate in odontoblasts, and weak in outer enamel epithelium and inner enamel epithelium/ameloblasts.

Structure	Level	Pattern	Image	Note
TS28: dental lamina	Weak
TS28: oral epithelium	Weak
TS28: stellate reticulum	Weak
TS28: stratum intermedium	Weak
TS28: molar	Present	Regionally restricted		Expression was strong in odontoblasts and weak in outer enamel epithelium.