Antibody:	YCC2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS8: extraembryonic ectoderm	Strong		1A
TS8: parietal endoderm	Strong		1A
TS8: primitive endoderm	Strong		1A

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	1A	Expression in the surrounding decidualized endometrium.
TS28: endometrium	Present	Regionally restricted	1A	Expression in the surrounding decidualized endometrium.
TS28: endometrium glandular epithelium	Absent		1A	No expression in endometrial glands or stroma distant from the implantation site.
TS28: endometrium stroma	Absent		1A	No expression in endometrial glands or stroma distant from the implantation site.

Structure	Level	Pattern	Image	Note
TS16: somite	Present	Regionally restricted	1B

Structure	Level	Pattern	Image	Note
TS20: mesonephros	Absent		2A

Structure	Level	Pattern	Image	Note
TS21: ureteric tip	Weak	Regionally restricted	2B	Low expression in the ureteric bud.
TS21: primitive collecting duct	Weak	Regionally restricted	2B	Low-intensity punctate granular cytoplasmic expression in ureteric vesicles.
TS21: mesenchyme of metanephros	Absent		2B	No detectable expression in the condensing mesenchyme.
TS21: comma-shaped body	Absent		2B
TS21: renal vesicle	Absent		2B
TS21: s-shaped body	Absent		2B

Structure	Level	Pattern	Image	Note
TS24: cortical renal tubule	Strong	Regionally restricted	2C	In maturing nephrons of inner cortex, strong basolateral expression in distal tubules.
TS24: maturing nephron	Strong	Regionally restricted	2C	In maturing nephrons of inner cortex, strong basolateral expression in distal tubules.
TS24: cortical early proximal tubule	Weak	Regionally restricted	2C	Low level punctate cytoplasmic expression in proximal tubules.
TS24: medullary collecting duct	Absent		2C	No expression in medullary collecting tubules
TS24: maturing glomerular tuft	Absent		2C	No expression in glomeruli, interstitium or interstitial capillaries.
TS24: mesenchyme of metanephros	Absent		2C	No expression in glomeruli, interstitium or interstitial capillaries.

Structure	Level	Pattern	Image	Note
TS27: renal cortex	Present	Regionally restricted	2D	Authors report segment specific expression in inner cortex and medulla.
TS27: renal medulla	Present	Regionally restricted	2D	Authors report segment specific expression in inner cortex and medulla.

Structure	Level	Pattern	Image	Note
TS27: renal distal tubule	Present	Regionally restricted	2E	Expression in maturing distal tubules of the inner cortex.
TS27: nephrogenic zone	Absent		2E

Structure	Level	Pattern	Image	Note
TS27: loop of Henle distal straight tubule	Present	Regionally restricted	2F	Expression in medullary thick ascending limbs.
TS27: medullary collecting duct	Absent		2F

Structure	Level	Pattern	Image	Note
TS28: nephron	Present	Regionally restricted	3A	Expression limited to distal nephron segments of cortex, thick ascending limbs of inner stripe of outer medulla, and collecting tubules of papillary tip.
TS28: papillary duct	Present	Regionally restricted	3A	Expression limited to distal nephron segments of cortex, thick ascending limbs of inner stripe of outer medulla, and collecting tubules of papillary tip.
TS28: renal cortex	Present	Regionally restricted	3A	Expression limited to distal nephron segments of cortex, thick ascending limbs of inner stripe of outer medulla, and collecting tubules of papillary tip.
TS28: inner stripe of outer medulla	Present		3A	Expression limited to distal nephron segments of cortex, thick ascending limbs of inner stripe of outer medulla, and collecting tubules of papillary tip.
TS28: loop of Henle distal straight tubule	Present		3A	Expression limited to distal nephron segments of cortex, thick ascending limbs of inner stripe of outer medulla, and collecting tubules of papillary tip.

Structure	Level	Pattern	Image	Note
TS28: distal convoluted tubule	Strong	Regionally restricted	3B	In the cortex, strong basolateral expression in distal convoluted tubules and macula densa.
TS28: macula densa	Strong	Regionally restricted	3B	In the cortex, strong basolateral expression in distal convoluted tubules and macula densa.
TS28: renal proximal tubule	Weak	Regionally restricted	3B	Weaker punctate expression in proximal tubules.
TS28: glomerular tuft	Absent		3B

Structure	Level	Pattern	Image	Note
TS28: renal distal tubule	Present	Regionally restricted	3C	Distinctly basolateral expression.
TS28: cortical collecting duct	Present	Regionally restricted	3C	A thinner line of basal expression.

Structure	Level	Pattern	Image	Note
TS28: renal proximal tubule	Present	Regionally restricted	3D	Authors report delicate basolateral expression.

Structure	Level	Pattern	Image	Note
TS28: renal medulla	Present	Regionally restricted	3E	Abrupt transition at the junction of inner stripe of outer medulla and inner medulla, corresponding to the transition from expression in medullary thick ascending limbs to no expression in thin limbs of Henle.
TS28: inner stripe of outer medulla	Present		3E	Abrupt transition at the junction of inner stripe of outer medulla and inner medulla, corresponding to the transition from expression in medullary thick ascending limbs to no expression in thin limbs of Henle.
TS28: loop of Henle distal straight tubule	Present		3E	Abrupt transition at the junction of inner stripe of outer medulla and inner medulla, corresponding to the transition from expression in medullary thick ascending limbs to no expression in thin limbs of Henle.
TS28: loop of Henle, inner medullary portion	Absent		3E	Abrupt transition at the junction of inner stripe of outer medulla and inner medulla, corresponding to the transition from expression in medullary thick ascending limbs to no expression in thin limbs of Henle.

Structure	Level	Pattern	Image	Note
TS28: papillary duct	Strong	Regionally restricted	3F	Strong granular basolateral expression in collecting ducts of papillary tip.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present	Diffuse	4A	Earliest expression in mesenchymal tissue was seen diffuse expression in cardiac myocytes.

Structure	Level	Pattern	Image	Note
TS25: pulmonary artery	Strong	Regionally restricted	4B	Highest expression of any smooth muscle was found in the smooth muscle of the pulmonary arteries.
TS25: smooth muscle tissue	Strong	Regionally restricted	4B	Highest expression of any smooth muscle was found in the smooth muscle of the pulmonary arteries.
TS25: lung mesenchyme	Absent		4B	No expression in pulmonary parenchyma.

Structure	Level	Pattern	Image	Note
TS24: skeletal muscle	Present	Regionally restricted	4C	Granular expression in skeletal myocytes.

Structure	Level	Pattern	Image	Note
TS24: gland	Strong		4D	Strong expression in endocrine tissues, most intense in adrenal cortex.
TS24: adrenal cortex	Very strong		4D	Strong expression in endocrine tissues, most intense in adrenal cortex.

Structure	Level	Pattern	Image	Note
TS28: pituitary gland	Moderate	Regionally restricted	4E	Moderate expression in both chromophilic and chromophobic cells of the pituitary gland.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Present		4F
TS28: exocrine pancreas	Absent		4F

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Strong		5A	Strong expression in chondrocytes of the vertebral bodies.

Structure	Level	Pattern	Image	Note
TS21: spinal ganglion	Moderate	Regionally restricted	5B	Moderate expression in paraspinal ganglia in a granular cytoplasmic pattern.

Structure	Level	Pattern	Image	Note
TS24: liver	Strong	Regionally restricted	5C	In the liver, high-intensity expression in nucleated red blood cell precursors.
TS24: liver parenchyma	Absent		5C

Structure	Level	Pattern	Image	Note
TS25: alimentary system epithelium	Present		5D	Expression in the epithelial cells of the gastrointestinal mucosa.

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Present			Expression in cardiac tissue, strongest at day E9.5.
TS15: neural tube	Present

Structure	Level	Pattern	Image	Note
TS19: neural tube	Absent

Structure	Level	Pattern	Image	Note
TS20: liver	Present	Regionally restricted
TS20: liver parenchyma	Absent

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Strong			High intensity expression in red blood cell precursors in the bone marrow after birth and into adult life.
TS28: renal distal tubule	Present	Regionally restricted		Authors state that the segment specific staining patterns in the proximal and distal tubules persisted throughout development and after birth.
TS28: renal proximal tubule	Present	Regionally restricted		Authors state that the segment specific staining patterns in the proximal and distal tubules persisted throughout development and after birth.
TS28: heart	Weak			Cardiac expression subsequently at lower levels that persisted postnatally.
TS28: adrenal cortex	Absent
TS28: alimentary system	Absent			No expression in gastrointestinal organs.
TS28: endometrium	Absent			No expression in nondecidualized endometrium and fallopian tube epithelium.
TS28: oviduct	Absent			No expression in nondecidualized endometrium and fallopian tube epithelium.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: germ cell of testis		Present			Expression in testicular germ cells and Sertoli cells.
TS28: Sertoli cell	Sertoli cell	Present			Expression in testicular germ cells and Sertoli cells.

Structure	Level	Pattern	Image	Note
TS28: corpus luteum	Present

Structure	Level	Pattern	Image	Note
TS28: pigmented retinal epithelium	Strong			Moderate to high expression in retinal pigment epithelium.
TS28: paranasal sinus	Strong	Regionally restricted		Moderate to high expression in ducts within the glandular epithelium of the paranasal sinuses, which had a distinctly basolateral distribution.