Antibody:	DNER antibody
Detection system:	Secondary antibody coupled to Alexa Fluor 568

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3B
TS28: cerebellum external granule cell layer	Weak	Regionally restricted	3B	Expression is in the inner-half of the external germinal layer.
TS28: cerebellum internal granule cell layer	Weak		3B

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3C	Expression is in the dendrites and cell bodies of Purkinje cells.
TS28: cerebellum granule cell layer	Weak		3C

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Weak	Regionally restricted	3D	Expression is in the pyramidal cell layer.
TS28: hippocampus CA3	Present	Regionally restricted	3D	Expression is in the pyramidal cell layer.
TS28: CA1 pyramidal cell layer	Weak		3D

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present		3E
TS28: hippocampus CA1	Present		3E	Expression is strong in the dendritic trees in the strata radiatum and lucidum.
TS28: hippocampus CA2	Present		3E	Expression is strong in the dendritic trees in the strata radiatum and lucidum.
TS28: hippocampus CA3	Present		3E	Expression is strong in the dendritic trees in the strata radiatum and lucidum.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	3F	Expression is in post-mitotic neurons in the subplate and cortical plate, absent in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Present	Regionally restricted	3G	Expression is in the apical dendrites and cell bodies of pyramidal neurons and is stronger in a subset of neurons scattered in each layer.
TS28: cortical layer II	Present	Regionally restricted	3G	Expression is in the apical dendrites and cell bodies of pyramidal neurons and is stronger in a subset of neurons scattered in each layer.
TS28: cortical layer III	Present	Regionally restricted	3G	Expression is in the apical dendrites and cell bodies of pyramidal neurons and is stronger in a subset of neurons scattered in each layer.
TS28: cortical layer IV	Present	Regionally restricted	3G	Expression is in the apical dendrites and cell bodies of pyramidal neurons and is stronger in a subset of neurons scattered in each layer.
TS28: cortical layer V	Present	Regionally restricted	3G	Expression is in the apical dendrites and cell bodies of pyramidal neurons and is stronger in a subset of neurons scattered in each layer.
TS28: cortical layer VI	Present	Regionally restricted	3G	Expression is in the apical dendrites and cell bodies of pyramidal neurons and is stronger in a subset of neurons scattered in each layer.