Probe:	Hoxd3 probe1
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	5B	Expression was detected in the neural tube and extended in the cranial direction up to and including rhombomere 5.
TS15: rhombomere 5	Present		5B
TS15: rhombomere 4	Absent		5B

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		5D

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	5E	Expression was detected in the neural tube and extended in the cranial direction up to and including rhombomere 5.
TS15: rhombomere 5	Present		5E
TS15: rhombomere 4	Absent		5E

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial arch	Present		6B
TS15: 3rd branchial arch	Present		6B

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		6D	Expression was detected along the entire spinal cord.
TS20: hindbrain	Present		6D	There was a sharp anterior limit of expression at the midbrain-hindbrain boundary.
TS20: midbrain	Absent		6D	There was a sharp anterior limit of expression at the midbrain-hindbrain boundary.
TS20: vertebral pre-cartilage condensation	Present	Regionally restricted	6D	The anterior border of expression in paraxial mesoderm derivatives was at the level of the first cervical prevertebra.
TS20: C1 vertebral cartilage condensation	Present		6D	The anterior border of expression in paraxial mesoderm derivatives was at the level of the first cervical prevertebra.
TS20: enteric nervous system	Present	Regionally restricted	6D	Expression was detected in the chain of dorsal ganglia in the gut.

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present		6F

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		7B	Expression was detected along the entire spinal cord.
TS20: hindbrain	Present		7B	There was a sharp anterior limit of expression at the midbrain-hindbrain boundary.
TS20: midbrain	Absent		7B	There was a sharp anterior limit of expression at the midbrain-hindbrain boundary.
TS20: enteric nervous system	Present	Regionally restricted	7B	Expression was detected in the chain of dorsal ganglia in the gut.
TS20: liver	Present		7B , 7D