Gene Expression Data
Assay Details
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Assay
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Reference:
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J:15850
Guillemot F, et al., Mammalian achaete-scute homolog 1 is required for the early development of olfactory and autonomic neurons. Cell. 1993 Nov 5;75(3):463-76
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4359514
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Gene symbol:
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Ncam1
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Gene name:
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neural cell adhesion molecule 1
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Antibody:
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anti-NCAM [H28.123]
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to alkaline phosphatase
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Results
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Specimen
wild type:
postnatal newborn
(more  )
Specimen
wild type:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: olfactory epithelium |
Present |
Regionally restricted |
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Expression is present in the neurons but not the basal and sustentacular cells.
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Specimen
mutant:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: olfactory epithelium |
Present |
Scattered |
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The olfactory epithelium is essentially devoid of expression, with the exception of a few areas where scattered immunoreactive cells could be observed.
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Specimen
wild type:
embryonic day 11.5-12.5
(more )
Specimen
wild type:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5-12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
mutant:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 11.5-12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: olfactory epithelium |
Present |
Regionally restricted |
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Expression is present in neurons. Expression is consistently lower in the mutant compared to wild type.
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Analysis Tools
