Probe:	IIa cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		6A
TS28: retina inner nuclear layer	Present		6A
TS28: retina outer nuclear layer	Present		6A
TS28: retina inner plexiform layer	Present	Single cells	6A	Expression is in some amacrine cells.

Structure	Level	Pattern	Image	Note
TS25: dorsal root ganglion	Very strong
TS25: myenteric nerve plexus	Very strong
TS25: spinal cord	Very strong
TS25: trigeminal ganglion	Very strong
TS25: brain ventricular layer	Strong	Regionally restricted		Expression is in postmitotic neurons, absent in proliferating neurons.
TS25: intestine epithelium	Weak
TS25: liver	Weak
TS25: lung	Weak
TS25: salivary gland	Weak

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Strong
TS28: retina inner nuclear layer	Strong
TS28: retina outer nuclear layer	Strong
TS28: retina inner plexiform layer	Strong	Single cells		Expression is in some amacrine cells.
TS28: central nervous system	Moderate	Regionally restricted		Expression is in all neurons except olfactory granule cells and most of the thalamus.
TS28: thalamus	Moderate	Regionally restricted
TS28: myenteric nerve plexus	Moderate
TS28: trigeminal ganglion	Moderate
TS28: cerebellum granule cell layer	Weak
TS28: salivary gland	Weak
TS28: small intestine crypt of lieberkuhn	Weak
TS28: thymus	Weak
TS28: main olfactory bulb granule cell layer	Absent