Probe:	Wif1 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Present	Regionally restricted	1A	Expression is in the mitral cell layer.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb mitral cell layer	Present		1B

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Present		1C

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Present		1D

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Present		1E

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Strong	Regionally restricted	2A	Expression is at an area near the cortical hem.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Strong	Graded	2B	Strong expression at the medial side of the preplate and ventricular zone to low at the lateral side.
TS20: cortical plate	Strong	Graded	2B	Strong expression at the medial side of the preplate and ventricular zone to low at the lateral side.
TS20: cerebral cortex	Strong	Regionally restricted	2B	Strong in the hippocampal plate.
TS20: cortical hem	Absent		2B	Expression absent in the cortical hem region.

Structure	Level	Pattern	Image	Note
TS22: cortical hem	Strong	Regionally restricted	2C	Expression is in both sides of the cortical hem.

Structure	Level	Pattern	Image	Note
TS24: cortical hem	Present	Regionally restricted	2D	Expression is in both sides of the cortical hem.
TS24: hippocampus	Present	Regionally restricted	2D	Expression is in mitotically active areas, the ventricular zone and subventricular zone.
TS24: cerebral cortex	Weak		2D

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Strong	Regionally restricted	2E	Expression is at an area near the cortical hem.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Strong	Graded	2F	Strong expression at the medial side of the preplate and ventricular zone to low at the lateral side.
TS20: cortical plate	Strong	Graded	2F	Strong expression at the medial side of the preplate and ventricular zone to low at the lateral side.
TS20: cerebral cortex	Strong	Regionally restricted	2F	Strong in the hippocampal plate.
TS20: cortical hem	Absent		2F	Expression absent in cortical hem region.
TS20: diencephalon	Present		2F

Structure	Level	Pattern	Image	Note
TS22: cortical hem	Strong	Regionally restricted	2G	Expression is in both sides of the cortical hem.
TS22: hippocampus	Present	Regionally restricted	2G	Expression is in mitotically active areas, the ventricular zone and subventricular zone.
TS22: diencephalon	Present		2G
TS22: cerebral cortex	Weak		2G

Structure	Level	Pattern	Image	Note
TS24: cortical hem	Present	Regionally restricted	2P , 2H	Expression is in both sides of the cortical hem.
TS24: hippocampus	Present	Regionally restricted	2P , 2H	Expression is in mitotically active areas, the ventricular zone and subventricular zone.
TS24: epithalamus	Strong		2P , 2H	Expression is in the medial habenular nucleus.
TS24: diencephalon	Present		2H , 2P
TS24: cerebral cortex	Weak		2P , 2H

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Strong	Regionally restricted	2I	Expression is at an area near the cortical hem.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Strong	Graded	2N , 2J	Strong expression at the medial side of the preplate and ventricular zone to low at the lateral side.
TS20: cortical plate	Strong	Graded	2N , 2J	Strong expression at the medial side of the preplate and ventricular zone to low at the lateral side.
TS20: cerebral cortex	Strong	Regionally restricted	2J , 2N	Strong in the hippocampal plate.
TS20: cortical hem	Absent		2N , 2J	Expression absent in cortical hem.
TS20: thalamus	Present	Regionally restricted	2J	Expression is in the lateral geniculate nucleus.
TS20: diencephalon	Present		2J

Structure	Level	Pattern	Image	Note
TS22: cortical hem	Strong	Regionally restricted	2O , 2K	Expression is in both sides of the cortical hem.
TS22: hippocampus	Present	Regionally restricted	2O , 2K	Expression is in mitotically active areas, the ventricular zone and subventricular zone.
TS22: diencephalon	Present		2K , 2O
TS22: pretectum	Present		2K , 2O
TS22: superior colliculus	Present		2K , 2O
TS22: cerebral cortex	Weak		2K , 2O

Structure	Level	Pattern	Image	Note
TS24: cortical hem	Present	Regionally restricted	2L	Expression is in both sides of the cortical hem.
TS24: hippocampus	Present	Regionally restricted	2L	Expression is in mitotically active areas, the ventricular zone and subventricular zone.
TS24: midbrain	Present	Regionally restricted	2L	Expression is in the pretectum.
TS24: diencephalon	Present		2L
TS24: superior colliculus	Present		2L
TS24: thalamus	Present	Regionally restricted	2L	Expression is in the medial geniculate nucleus.
TS24: cerebral cortex	Weak		2L

Structure	Level	Pattern	Image	Note
TS20: optic chiasma	Strong		2M

Structure	Level	Pattern	Image	Note
TS27: medial habenular nucleus	Strong		3A , 3C
TS27: fimbria hippocampus	Strong	Regionally restricted	3B , 3A	Expression is in the lateral edge of fimbria.
TS27: cerebral cortex	Absent		3C , 3B , 3A

Structure	Level	Pattern	Image	Note
TS28: medial habenular nucleus	Strong		3D , 3F
TS28: fimbria hippocampus	Strong	Regionally restricted	3E , 3D	Expression is in the lateral edge of fimbria.
TS28: cerebral cortex	Absent		3E , 3F , 3D

Structure	Level	Pattern	Image	Note
TS28: medial habenular nucleus	Strong		3H , 3G
TS28: hippocampus	Weak		3G , 3H
TS28: cerebral cortex	Absent		3G , 3H

Structure	Level	Pattern	Image	Note
TS28: medial habenular nucleus	Strong		3I , 3J
TS28: cerebral cortex	Absent		3I , 3J
TS28: hippocampus	Absent		3I , 3J

Structure	Level	Pattern	Image	Note
TS18: midbrain	Strong	Regionally restricted	4B , 4A	Expression is in the pineal recess, superior colliculus and mesencephalic tegmentum.
TS18: metencephalon basal plate	Present		4A

Structure	Level	Pattern	Image	Note
TS20: pons	Present		4C
TS20: midbrain	Present	Regionally restricted	4C	Expression is in the superior colliculus and the pretectum.

Structure	Level	Pattern	Image	Note
TS22: pretectum	Present		4D
TS22: superior colliculus	Present		4D

Structure	Level	Pattern	Image	Note
TS24: midbrain	Present	Regionally restricted	4E	Expression is in the pretectum.
TS24: midbrain tegmentum	Present		4E
TS24: pons	Present		4E
TS24: superior colliculus	Present		4E

Structure	Level	Pattern	Image	Note
TS27: pretectum	Present		4F
TS27: superior colliculus	Present		4F

Structure	Level	Pattern	Image	Note
TS28: medial dorsal thalamic nucleus	Present		4G
TS28: pretectum	Present		4G
TS28: superior colliculus	Present		4G

Structure	Level	Pattern	Image	Note
TS28: pretectum	Present		4H

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present	Regionally restricted	5B , 5C , 5A	Expression is in the third differentiating zone of cerebellar Purkinje neurons, the cerebellar germinal trigone and the precerebellar neuroepithelium.

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Present	Regionally restricted	5E , 5D	Expression is in the cerebellar germinal trigone.

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Present	Regionally restricted	5G , 5F	Expression is in the cerebellar germinal trigone.
TS27: cerebellum Purkinje cell layer	Present		5F , 5G

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5H

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5I
TS28: facial nucleus	Present		5I
TS28: trigeminal V spinal sensory nucleus	Present		5I
TS28: vestibular nucleus	Present		5I

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5J

Structure	Level	Pattern	Image	Note
TS18: future spinal cord	Present	Regionally restricted	6A	Expression is in the dorsal spinal cord and is weak in the ventral-lateral part.
TS18: dorsal root ganglion	Strong		6A
TS18: neural tube roof plate	Strong		6A

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Strong		6B
TS20: spinal cord roof plate	Strong		6B

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Present	Regionally restricted	6C	Expression is in a wide region of the ventral spinal cord.
TS24: dorsal root ganglion	Strong		6C

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Present	Regionally restricted	6E , 6D	Expression is in cells in the ventral-lateral part.

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Weak			Weak expression was found from P4 to adult.

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Weak