Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:135212
Liu WB, et al., Differential expression of the catalytic subunits for PP-1 and PP-2A and the regulatory subunits for PP-2A in mouse eye. Mol Vis. 2008;14:762-73
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:4353262
|
|
Gene symbol:
|
Ppp2r1b
|
|
Gene name:
|
protein phosphatase 2, regulatory subunit A, beta
|
|
Antibody:
|
Anti-PP-2A-A
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
Assay notes:
|
The antibody recognizes both the alpha and beta isoforms of PP-2A-A; see assay MGI:4353259.
|
|
|
Results
|
Specimen
7C:
embryonic day 15.5
(more  )
Specimen
7C:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cornea |
Strong |
|
7C
|
The cornea had the most intensive immunofluorescence signal.
|
| TS23: pigmented retinal epithelium |
Present |
|
7C
|
Expression in the pigment layer of the retina was the second strongest immunofluorescence signal after the cornea.
|
| TS23: lens fiber |
Present |
|
7C
|
Expression levels in the neuro-retinal layer was about the same as that in the lens fiber cells.
|
| TS23: neural retina |
Present |
|
7C
|
Expression levels in the neuro-retinal layer was about the same as that in the lens fiber cells.
|
| TS23: lens epithelium |
Present |
|
7C
|
The lowest fluorescence signal was found in the lens epithelial cells.
|
|
Analysis Tools