Gene Expression Data
Assay Details
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Assay
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Reference:
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J:150374
Garcia MI, et al., LGR5 deficiency deregulates Wnt signaling and leads to precocious Paneth cell differentiation in the fetal intestine. Dev Biol. 2009 Jul 1;331(1):58-67
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:4352740
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Gene symbol:
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Lgr5
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Gene name:
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leucine rich repeat containing G protein coupled receptor 5
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fixative: 0.5% paraformaldehyde, 2.5% glutaraldehyde.
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Results
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Specimen
1B HE:
embryonic day 18.5; Lgr5tm1Ah/Lgr5 +
(more  )
Specimen
1B HE:
(close  )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: ileum |
Present |
Regionally restricted |
1B HE
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Expression was detected exclusively in cells clustered in the intervillus region.
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Specimen
1B KO:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: ileum |
Present |
Regionally restricted |
1B KO
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Expression was detected exclusively in cells clustered in the intervillus region.
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Specimen
1C HE left:
embryonic day 18.5; Lgr5tm1Ah/Lgr5 +
(more )
Specimen
1C HE left:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: duodenum |
Present |
Regionally restricted |
1C left
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Expression was detected in the intervillus region.
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Specimen
1C HE right:
embryonic day 18.5; Lgr5tm1Ah/Lgr5 +
(more )
Specimen
1C HE right:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: colon |
Present |
Regionally restricted |
1C right
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A few positive cells were detected in the crypts. Higher magnification was shown in the inset.
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Specimen
1D HE:
embryonic day 15.5; Lgr5tm1Ah/Lgr5 +
(more )
Specimen
1D HE:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: ileum |
Present |
Regionally restricted |
1D HE
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Higher magnification was shown in the inset. Expression was detected in the ileal epithelium.
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Specimen
1D KO:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: ileum |
Present |
Regionally restricted |
1D KO
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Higher magnification was shown in the inset. Expression was detected in the ileal epithelium.
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Specimen
2A HE:
embryonic day 18.5; Lgr5tm1Ah/Lgr5 +
(more )
Note: Double labeled: blue - Lgr5; brown - BrdU.
Specimen
2A HE:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Lgr5; brown - BrdU.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: ileum |
Present |
Regionally restricted |
2A HE
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Expression was detected in the intervillus region.
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Specimen
2A KO:
embryonic day 18.5; Lgr5tm1Ah/ Lgr5tm1Ah
(more )
Note: Double labeled: blue - Lgr5; brown - BrdU.
Specimen
2A KO:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Lgr5; brown - BrdU.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: ileum |
Present |
Regionally restricted |
2A KO
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Expression was detected in the intervillus region.
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Specimen
4C right:
embryonic day 18.5; Lgr5tm1Ah/ Lgr5tm1Ah
(more )
Note: Double labeled: blue - Lgr5; brown - Lendrum's stain.
Specimen
4C right:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Lgr5; brown - Lendrum's stain.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: ileum |
Present |
Regionally restricted |
4C right
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Expression was detected in Paneth cells.
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Analysis Tools
