Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tcirg1tm1.1Ywd
Name:	T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3; targeted mutation 1.1, Yoh Wada
MGI ID:	MGI:3852639
Synonyms:	a3-GFP, Tcirg1floxGFP
Gene:	Tcirg1  Location: Chr19:3946050-3957133 bp, - strand  Genetic Position: Chr19, 3.62 cM
Alliance:	Tcirg1tm1.1Ywd page

Germline Transmission:	Earliest citation of germline transmission: J:150327
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout, Reporter) Mutation:    Insertion   Mutation details: This allele contains a single Frt site and a single loxP site in intron 13 and the EGFP coding sequence, followed by another loxP site, inserted into the last exon. The modified gene encodes a fusion protein in which the C-terminal of TCIRG1 is joined to the N-terminal of EGFP by the linker sequence AALELVDPPGSIAT. EGFP fluorescence and the immunofluorescence signal for the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, are colocalized in the late endocytic compartments of mutant peritoneal macrophages. Cre recombinase excision of the loxP-flanked DNA segment generates a null allele. (J:150327)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Tcirg1 Mutation:	35 strains or lines available

Original:	J:150327 Sun-Wada GH, et al., Direct recruitment of H+-ATPase from lysosomes for phagosomal acidification. J Cell Sci. 2009 Jul 15;122(Pt 14):2504-13
All:	4 reference(s)