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Gene Expression Data
Assay Details
Assay
Reference: J:146993 Wyrwoll CS, et al., Altered placental function of 11beta-hydroxysteroid dehydrogenase 2 knockout mice. Endocrinology. 2009 Mar;150(3):1287-93
Assay type: RT-PCR
MGI Accession ID: MGI:3851588
Gene symbol: Vegfa
Gene name: vascular endothelial growth factor A
Probe: Mm00437304_m1
Assay notes: Real-time quantitative PCR. Placental zones were separated by blunt dissection and the labyrinth zone was used for analysis.
Results Image: 4A
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
E15 +/+ E14.5 (a) TS22: extraembryonic component
TS28: placenta 		Present 1 µg; total RNA involves: 129P2/OlaHsd * C57BL/6J Not Specified
E15 +/- E14.5 (a) TS22: extraembryonic component
TS28: placenta 		Present 1 µg; total RNA B6.129P2-Hsd11b2tm1Yko Hsd11b2tm1Yko/Hsd11b2+Not Specified
E15 -/- E14.5 (a) TS22: extraembryonic component
TS28: placenta 		Present (b) 1 µg; total RNA B6.129P2-Hsd11b2tm1Yko Hsd11b2tm1Yko/Hsd11b2tm1YkoNot Specified
E18 +/+ E17.5 (a) TS25: extraembryonic component
TS28: placenta 		Strong (c) 1 µg; total RNA involves: 129P2/OlaHsd * C57BL/6J Not Specified
E18 +/- E17.5 (a) TS25: extraembryonic component
TS28: placenta 		Strong (c) 1 µg; total RNA B6.129P2-Hsd11b2tm1Yko Hsd11b2tm1Yko/Hsd11b2+Not Specified
E18 -/- E17.5 (a) TS25: extraembryonic component
TS28: placenta 		Weak (d) 1 µg; total RNA B6.129P2-Hsd11b2tm1Yko Hsd11b2tm1Yko/Hsd11b2tm1YkoNot Specified
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression is similar as in wild-type.
(c) There was an 8-fold increase in expression compared to E15.
(d) Expression decreased 3-fold compared to wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory