Gene Expression Data
Assay Details
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Assay
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Reference:
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J:51992
Tsuda T, et al., Differential expression of flectin in the extracellular matrix and left-right asymmetry in mouse embryonic heart during looping stages. Dev Genet. 1998;23(3):203-14
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3850501
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Gene symbol:
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Myh10
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Gene name:
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myosin, heavy polypeptide 10, non-muscle
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Antibody:
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mAb F22
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Detection system:
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Secondary antibody coupled to Cy3
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Assay notes:
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The antibody recognizes two proteins; both have been annotated.
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Results
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Specimen
Not shown:
embryonic day 8.0
(more  )
Note: Embryos were embedded in araldite.
Specimen
Not shown:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: cardiac muscle tissue |
Present |
Regionally restricted |
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Expression is in the myocardial extracellular matrix.
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Specimen
1A:
embryonic day 8.5
(more )
Note: Embryos were embedded in araldite.
Specimen
1A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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Specimen
1B:
embryonic day 8.5
(more )
Note: Embryos were embedded in araldite.
Specimen
1B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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Specimen
1C:
embryonic day 8.5
(more )
Note: Embryos were embedded in araldite.
Specimen
1C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: cardiac jelly |
Present |
Regionally restricted |
1C
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Expression is in the extracellular matrix.
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| TS13: cardiac muscle tissue |
Present |
Regionally restricted |
1C
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Expression is in the extracellular matrix in the myocardium.
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Specimen
3A:
embryonic day 8.75
(more )
Note: Embryos were embedded in araldite.
Specimen
3A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.75
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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Specimen
3B:
embryonic day 8.75
(more )
Note: Embryos were embedded in araldite.
Specimen
3B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.75
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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Specimen
3C:
embryonic day 8.75
(more )
Note: Embryos were embedded in araldite.
Specimen
3C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.75
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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Specimen
4A,B:
embryonic day 9.0
(more )
Note: Embryos were embedded in araldite.
Specimen
4A,B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: heart |
Strong |
Regionally restricted |
4B
,
4A
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Expression is in the inner curvature of the looping heart and on the left side of the distal tubular heart.
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| TS14: cardiac muscle tissue |
Present |
Regionally restricted |
4B
,
4A
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Expression is in the extracellular matrix of the myocardium.
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| TS14: cardiac jelly |
Present |
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4A
,
4B
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Specimen
Not shown:
embryonic day 9.0
(more )
Note: Embryos were embedded in araldite.
Specimen
Not shown:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: outflow tract |
Present |
Regionally restricted |
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There is increased right-sided expression in the outflow tract.
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Specimen
5:
embryonic day 9.0
(more )
Note: Embryos were embedded in araldite.
Specimen
5:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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Specimen
6A:
embryonic day 9.5
(more )
Note: Embryos were embedded in araldite.
Specimen
6A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: primitive ventricle |
Present |
Regionally restricted |
6A
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Expression is localized at the left side of common ventricle.
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| TS15: outflow tract |
Present |
Graded |
6A
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There is increased flectin expression on the right side of the outflow tract.
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| TS15: endocardial lining |
Present |
Regionally restricted |
6A
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Expression is in the lining of the endocardial endothelium.
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Specimen
6B:
embryonic day 9.5
(more )
Note: Embryos were embedded in araldite.
Specimen
6B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: primitive ventricle |
Present |
Regionally restricted |
6B
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Expression is localized at the left side of common ventricle.
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Specimen
6C:
embryonic day 9.5
(more )
Note: Embryos were embedded in araldite.
Specimen
6C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
|
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
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Resin/plastic
|
|
Note:
|
Embryos were embedded in araldite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: primitive ventricle |
Present |
Regionally restricted |
6C
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Expression is localized at the left side of common ventricle.
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Specimen
Not shown:
embryonic day 9.5
(more )
Note: Embryos were embedded in araldite.
Specimen
Not shown:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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Specimen
7:
embryonic day 11.5
(more )
Note: Embryos were embedded in araldite.
Specimen
7:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: visceral pericardium |
Present |
Regionally restricted |
7
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Expression is in the epicardium of the right ventricular wall and atrial wall.
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| TS19: heart |
Weak |
Regionally restricted |
7
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Flectin is also mildly expressed in the trabeculated myocardial extracellular matrix in the right ventricular sinus.
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Specimen
8A:
embryonic day 9.5; Invsinv/ Invsinv
(more )
Note: Embryos were embedded in araldite.
Specimen
8A:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Invsinv/Invsinv
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: outflow tract |
Present |
Regionally restricted |
8A
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Expression is predominantly in the left side of outflow tract, which is the mirror image of the normal flectin expression pattern.
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Specimen
8B:
embryonic day 9.5; Invsinv/ Invsinv
(more )
Note: Embryos were embedded in araldite.
Specimen
8B:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Invsinv/Invsinv
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embryos were embedded in araldite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: heart |
Present |
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8B
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Expression is the right side of ventricular tubular heart.
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| TS15: cardiac jelly |
Present |
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8B
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Expression in the cardiac jelly is quite similar to that of normal mouse embryos.
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