Summary | Mutation origin | Mutation description | Expression | Recombinase activity | Phenotypes | Find Mice (IMSR) | Notes | References

Symbol:	Lmx1btm2(cre/ERT2)Rjo
Name:	LIM homeobox transcription factor 1 beta; targeted mutation 2, Randy L Johnson
MGI ID:	MGI:3849050
Synonyms:	Lmx1b-IREScreERT2
Gene:	Lmx1b  Location: Chr2:33450977-33530620 bp, - strand  Genetic Position: Chr2, 22.48 cM
Alliance:	Lmx1btm2(cre/ERT2)Rjo page

Germline Transmission:	Earliest citation of germline transmission: J:149241
Parent Cell Line:	PC3 (ES Cell)
Strain of Origin:	129S4/SvJae-Tg(Prm-cre)70Og

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Lmx1btm2(cre/ERT2)Rjo expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Lmx1b (MGI:1100513)   Mutation details: An IREScreERT2 cassette followed by a loxP flanked PGK-neo cassette was inserted into the 3'UTR of the Lmx1b locus by homologous recombination in PC3 ES cells. Cre mediated recombination subsequently removed the PGK-neo cassette upon transmission from male chimeric mice. The protamine-cre transgene contributed by the PC3 cells was separated from the knock-in allele by crossing germline male mutants with C57BL/6 mice. (J:149241)

Strategy for generating the Lmx1btm2(cre/ERT2)Rjo and Lmx1btm3(cre/ERT2)Rjo alleles

Activity:	Tissue activity of this recombinase allele
Driver:	Lmx1b (mouse) Summary of all recombinase alleles driven by Lmx1b. Comparative matrix view of recombinase activities Alliance mouse Lmx1b gene page

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

3 assay results

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Lmx1b Mutation:	16 strains or lines available

Original:	J:149241 Qiu Q, et al., Lmx1b-expressing cells in the mouse limb bud define a dorsal mesenchymal lineage compartment. Genesis. 2009 Apr;47(4):224-33
All:	3 reference(s)