Tg(Nkx2-5-GFP)21Rjs
Transgene Detail
|
|
| Symbol: |
Tg(Nkx2-5-GFP)21Rjs |
| Name: |
transgene insertion 21, Robert J Schwartz |
| MGI ID: |
MGI:3848018 |
| Synonyms: |
Nkx2-5 BAC-GFP |
| Transgene: |
Tg(Nkx2-5-GFP)21Rjs Location: unknown
|
| Alliance: |
Tg(Nkx2-5-GFP)21Rjs page
|
|
|
|
| Transgene Type: |
|
Transgenic (Reporter) |
| Mutation: |
|
Insertion
|
| |
|
Mutation details: A BAC clone containing the Nkx2-5 locus was modified by homologous recombination in E. coli. A targeting vector containing the hrGFP reporter, FRT-flanked kanamycin/TK selection cassette, and Nkx2-5 3' and 5' homology arms was used to replace exon 1 and intron 1 of the Nkx2-5 locus via recombination in red-alpha and red-beta recombinase expressing cells. The selection cassette was excised from recombinant clones by induction of lambda PR-Flp in the bacterial cells. The recombined BAC DNA was microinjected into C57 mice. Four lines of transgenic mice were generated.
(J:83506)
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
|
| Original: |
J:83506 Chi X, et al., Expression of Nkx2-5-GFP bacterial artificial chromosome transgenic mice closely resembles endogenous Nkx2-5 gene activity. Genesis. 2003 Apr;35(4):220-6 |
| All: |
2 reference(s) |
|
Analysis Tools
