Tg(Cela1-cre/ERT)1Lgdn
Transgene Detail
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| Symbol: |
Tg(Cela1-cre/ERT)1Lgdn |
| Name: |
transgene insertion 1, Craig Logsdon |
| MGI ID: |
MGI:3848026 |
| Synonyms: |
BAC-Ela-CreErT, Ela-Cre-ERT2 |
| Transgene: |
Tg(Cela1-cre/ERT)1Lgdn Location: unknown
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| Alliance: |
Tg(Cela1-cre/ERT)1Lgdn page
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| Transgene Type: |
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Transgenic (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tg(Cela1-cre/ERT)1Lgdn expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Cela1 (MGI:95314) |
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Mutation details: A mouse BAC (clone RP23-359O19, size 222 kb) carrying the intact elastase I (Cela1) gene was used. The BAC was modified to express CreERT. For recombination-mediated targeting, arms homologous to the sequence of elastase I gene between the last exon and the polyA signal (in lower case) were added to either side of the IRES-Cre-ERT-frt-Kan-frt cassette by PCR. Using homologous recombination, the IRES-Cre-ERT-frt-Kan-frt cassette was inserted into the elastase I gene. The Kan selection marker was then removed by flp-mediated recombination. two BAC-Cela1-CreERT founders were identified by genotyping. Q-PCR based analysis indicated the presence of 2-3 copies of the transgene in BAC-Cela1-CreERT founders.
(J:148748)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Cela1
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:148748 Ji B, et al., Robust acinar cell transgene expression of CreErT via BAC recombineering. Genesis. 2008 Aug;46(8):390-5 |
| All: |
23 reference(s) |
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Analysis Tools