Gene Expression Data
Assay Details
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Assay
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Reference:
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J:33697
Gibson-Brown JJ, et al., Evidence of a role for T-box genes in the evolution of limb morphogenesis and the specification of forelimb/hindlimb identity. Mech Dev. 1996 May;56(1-2):93-101
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3846937
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Gene symbol:
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Tbx2
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Gene name:
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T-box 2
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Probe:
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Tbx2 cDNA5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1A:
embryonic day 9.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Regionally restricted |
1A
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Expression at the posterior margin of the forelimb bud.
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Specimen
1B:
embryonic day 10.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
1B
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Expression in the anterior and posterior proximal mesenchyme of both forelimb and hindlimb buds.
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| TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
1B
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Expression in the anterior and posterior proximal mesenchyme of both forelimb and hindlimb buds.
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Specimen
1C:
embryonic day 11.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
1C
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Expression in anterior mesenchyme restricted to the proximal half of the limb margin. Expression extends in a broad stripe from the body wall to the distal tip of the autopod.
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| TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
1C
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Expression in anterior mesenchyme restricted to the proximal half of the limb margin. Expression extends in a broad stripe from the body wall to the distal tip of the autopod.
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Specimen
1D:
embryonic day 12.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1E:
embryonic day 13.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2A:
embryonic day 11.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2B:
embryonic day 11.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools