Mthfr RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:147857 Liu SP, et al., Glycine N-methyltransferase-/- mice develop chronic hepatitis and glycogen storage disease in the liver. Hepatology. 2007 Nov;46(5):1413-25
Assay type:	RT-PCR
MGI Accession ID:	MGI:3846859
Gene symbol:	Mthfr
Gene name:	methylenetetrahydrofolate reductase

Probe:	Mthfr-pA, Mthfr-pB
Assay notes:	Real-time polymerase chain reaction analysis.

Results

Image: 3_Mthfr

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
WTM	P	TS28: liver	Present (a)	5 µg; total RNA	Not Specified		Male
KOM	P	TS28: liver	Present (b)	5 µg; total RNA	involves: 129 * C57BL/6	Gnmt^tm1Ymac/Gnmt^tm1Ymac	Male
WTF	P	TS28: liver	Present (a)	5 µg; total RNA	Not Specified		Female
KOF	P	TS28: liver	Present (b)	5 µg; total RNA	involves: 129 * C57BL/6	Gnmt^tm1Ymac/Gnmt^tm1Ymac	Female

Notes:
(a) Expression level in male wild type mice was significantly lower than in female wild type mice.
(b) Expression level in the mutant was significantly down-regulated compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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