Probe:	PTH/PTHrPR probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS7: extraembryonic visceral endoderm	Present		3A	Representative of 6 different embryos, all showing identical expression patterns.
TS7: parietal endoderm	Present		3A	Representative of 6 different embryos, all showing identical expression patterns.

Structure	Level	Pattern	Image	Note
TS7: extraembryonic visceral endoderm	Present		3B	Representative of 6 different embryos, all showing identical expression patterns.
TS7: parietal endoderm	Present		3B	Representative of 6 different embryos, all showing identical expression patterns.

Structure	Level	Pattern	Image	Note
TS9: parietal endoderm	Strong	Regionally restricted	4A/B	Especially high expression in the marginal zone in which new parietal endoderm cells are formed.
TS9: extraembryonic visceral endoderm	Present		4A/B
TS9: extraembryonic ectoderm	Absent		4A/B
TS9: primitive endoderm	Absent		4A/B

Structure	Level	Pattern	Image	Note
TS9: parietal endoderm	Strong	Regionally restricted	4C/D	Especially high expression in the marginal zone in which new parietal endoderm cells are formed.
TS9: extraembryonic visceral endoderm	Present		4C/D
TS9: extraembryonic ectoderm	Absent		4C/D
TS9: primitive endoderm	Absent		4C/D

Structure	Level	Pattern	Image	Note
TS9: parietal endoderm	Strong	Regionally restricted	4E/F	Especially high expression in the marginal zone in which new parietal endoderm cells are formed.
TS9: extraembryonic visceral endoderm	Present		4E/F
TS9: primitive endoderm	Absent		4E/F
TS9: extraembryonic ectoderm	Weak	Regionally restricted	4E/F	Low levels of expression in extraembryonic ectoderm cells near the primitive streak.

Structure	Level	Pattern	Image	Note
TS9: parietal endoderm	Strong	Regionally restricted	4G/H	Especially high expression in the marginal zone in which new parietal endoderm cells are formed.
TS9: extraembryonic visceral endoderm	Present		4G/H
TS9: primitive endoderm	Absent		4G/H
TS9: embryo	Absent		4G/H	No expression in the epiblast.

Structure	Level	Pattern	Image	Note
TS11: extraembryonic visceral endoderm	Strong		4I/K
TS11: parietal endoderm	Strong		4I/K
TS11: embryo	Absent		4I/K	Authors state there was no expression in all other cell layers.
TS11: extraembryonic ectoderm	Absent		4I/K	Authors state there was no expression in all other cell layers.
TS11: extraembryonic mesoderm	Absent		4I/K	Authors state there was no expression in all other cell layers.

Structure	Level	Pattern	Image	Note
TS11: extraembryonic visceral endoderm	Strong		4L/M
TS11: parietal endoderm	Strong		4L/M
TS11: embryo	Absent		4L/M	Authors state there was no expression in all other cell layers.
TS11: extraembryonic ectoderm	Absent		4L/M	Authors state there was no expression in all other cell layers.
TS11: extraembryonic mesoderm	Absent		4L/M	Authors state there was no expression in all other cell layers.

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Strong	Regionally restricted	5A/B	High levels of expression in the parietal endoderm.
TS13: yolk sac endoderm	Absent		5A/B	No expression in the visceral endoderm component of the yolk sac.

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Strong	Regionally restricted	5C/D	High levels of expression in the parietal endoderm.
TS13: yolk sac endoderm	Absent		5C/D	No expression in the visceral endoderm component of the yolk sac.

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Present	Regionally restricted	5E/F	Authors state that there was no expression in the visceral endoderm component of the yolk sac, except at the marginal zone near the ectoplacental cone, where visceral endoderm remains in contact with parietal endoderm.

Structure	Level	Pattern	Image	Note
TS15: extraembryonic component	Strong	Regionally restricted	5G/H	High levels of expression in the parietal endoderm.
TS15: gut	Strong	Regionally restricted	5G/H	Authors report high expression in the intestine.

Structure	Level	Pattern	Image	Note
TS17: extraembryonic component	Present	Regionally restricted		Expression in the parietal endoderm.
TS17: lung	Weak			Faint expression in lung buds.

Structure	Level	Pattern	Image	Note
TS15: gut	Strong	Regionally restricted	6A/B	Authors report high expression in the intestine.

Structure	Level	Pattern	Image	Note
TS19: surface ectoderm	Present		6C/D	Authors report expression in the skin.
TS19: skeleton	Present	Regionally restricted	6C/D	Expression first detected in developing bones in the clivus (cartilage primordium of the basioccipital bone).

Structure	Level	Pattern	Image	Note
TS20: skin	Present		6E/F
TS20: axial skeleton	Present	Regionally restricted	6E/F	Expression in the clavicle, vertebral column, ribs and other long bones.
TS20: skeleton	Present	Regionally restricted	6E/F	Expression in the clavicle, vertebral column, ribs and other long bones.

Structure	Level	Pattern	Image	Note
TS21: lung mesenchyme	Present		6G/H	Expression in lung buds, restricted to the mesenchyme beneath the lung epithelium.
TS21: lung epithelium	Absent		6G/H	Expression in lung buds, restricted to the mesenchyme beneath the lung epithelium.
TS21: skin	Present		6G/H
TS21: axial skeleton	Present	Regionally restricted	6G/H	Expression in the clavicle, vertebral column, ribs and other long bones.
TS21: skeleton	Present	Regionally restricted	6G/H	Expression in the clavicle, vertebral column, ribs and other long bones.
TS21: clavicle pre-cartilage condensation	Present		6G/H	Expression in the clavicle, vertebral column, ribs and other long bones.

Structure	Level	Pattern	Image	Note
TS22: lung	Absent		6J/K	Authors report expression in lungs disappears after 13.5 days p.c..
TS22: skin	Present		6J/K
TS22: skeleton	Present	Regionally restricted	6J/K	Expression in the clavicle, vertebral column, ribs and other long bones.
TS22: clavicle cartilage condensation	Present		6J/K	Expression in the clavicle, vertebral column, ribs and other long bones.
TS22: rib cartilage condensation	Present		6J/K	Expression in the clavicle, vertebral column, ribs and other long bones.
TS22: vertebral cartilage condensation	Present		6J/K	Expression in the clavicle, vertebral column, ribs and other long bones.

Structure	Level	Pattern	Image	Note
TS23: lung	Absent		6L/M	Authors report expression in lungs disappears after 13.5 days p.c..
TS23: skin	Present		6L/M
TS23: skeleton	Present	Regionally restricted	6L/M	Expression in the clavicle, vertebral column, ribs and other long bones.
TS23: clavicle	Present		6L/M	Expression in the clavicle, vertebral column, ribs and other long bones.
TS23: rib	Present		6L/M	Expression in the clavicle, vertebral column, ribs and other long bones.
TS23: vertebra	Present		6L/M	Expression in the clavicle, vertebral column, ribs and other long bones.

Structure	Level	Pattern	Image	Note
TS17: midgut	Present		7A/B	Authors report expression restricted to a region probably belonging to the midgut.
TS17: gut epithelium	Present	Regionally restricted	7A/B	Expression restricted to intestine epithelium.
TS17: midgut mesenchyme	Absent		7A/B

Structure	Level	Pattern	Image	Note
TS20: intestine epithelium	Strong		7C/D	Authors report maximum expression at 12.5 days p.c..
TS20: intestine mesenchyme	Absent		7C/D

Structure	Level	Pattern	Image	Note
TS21: intestine epithelium	Weak		7E/F	Authors state expression slowly disappeared after 12.5 days p.c..
TS21: intestine mesenchyme	Absent		7E/F

Structure	Level	Pattern	Image	Note
TS22: intestine	Absent			Expression in intestine is no longer detectable.
TS22: skin	Strong	Regionally restricted		Expression in skin, particularly high in dermal regions near the hindlimb bud.

Structure	Level	Pattern	Image	Note
TS19: lung mesenchyme	Present		7G/H	Expression in lung buds, restricted to the mesenchyme beneath the lung epithelium.
TS19: lung epithelium	Absent		7G/H	Expression in lung buds, restricted to the mesenchyme beneath the lung epithelium.

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present	Regionally restricted	7J/K	Expression in lung buds, restricted to the mesenchyme beneath the lung epithelium. Highest expression in mesenchymal cells directly bordering epithelium cells.
TS20: lung epithelium	Absent		7J/K

Structure	Level	Pattern	Image	Note
TS21: lung mesenchyme	Present		7L/M	Expression in lung buds, restricted to the mesenchyme beneath the lung epithelium.
TS21: lung epithelium	Absent		7L/M	Expression in lung buds, restricted to the mesenchyme beneath the lung epithelium.

Structure	Level	Pattern	Image	Note
TS19: mesonephric tubule	Present		8A/B	Clear expression in the mesonephros, restricted to mesonephric tubuli.

Structure	Level	Pattern	Image	Note
TS20: mesonephric tubule	Present		8C/D	Clear expression in the mesonephros, restricted to mesonephric tubuli. Expression increases with development of the mesonephros.

Structure	Level	Pattern	Image	Note
TS22: renal medulla	Present	Regionally restricted	8E/F	Expression in the kidney, restricted to certain parts of the medulla in which renal tubuli are located; less intense than that seen in the mesonephros.

Structure	Level	Pattern	Image	Note
TS23: renal medulla	Present	Regionally restricted	8G/H	Expression in the kidney, restricted to certain parts of the medulla in which renal tubuli are located; less intense than that seen in the mesonephros.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Strong	Regionally restricted	8J/K	Expression particularly high in dermal regions near the hindlimb bud. Highest levels of expression in cells directly adjacent to the epidermis. No expression in the epidermis.
TS19: surface ectoderm	Strong	Regionally restricted	8J/K	Expression particularly high in dermal regions near the hindlimb bud. Highest levels of expression in cells directly adjacent to the epidermis. No expression in the epidermis.

Structure	Level	Pattern	Image	Note
TS20: dermis	Strong	Regionally restricted	8L/M	Expression particularly high in dermal regions near the hindlimb bud. Highest levels of expression in cells directly adjacent to the epidermis.
TS20: hindlimb	Strong	Regionally restricted	8L/M	Expression particularly high in dermal regions near the hindlimb bud. Highest levels of expression in cells directly adjacent to the epidermis.
TS20: epidermis	Absent		8L/M

Structure	Level	Pattern	Image	Note
TS23: dermis	Present	Regionally restricted	8N/O
TS23: epidermis	Absent		8N/O
TS23: vibrissa dermal component	Strong	Regionally restricted	8N/O	Expression particularly high in the snout, especially in the dermal sheath surrounding the vibrissae. Highest levels of expression in cells directly adjacent to the follicles.
TS23: vibrissa epidermal component	Absent		8N/O

Structure	Level	Pattern	Image	Note
TS19: rib pre-cartilage condensation	Absent		9A/B	No expression in condensing mesenchymal cells of vertebrae or ribs.
TS19: vertebral pre-cartilage condensation	Absent		9A/B	No expression in condensing mesenchymal cells of vertebrae or ribs.

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Strong		9C/D	Intense expression in mesenchymal cells that morphologically resemble chondrocytes.

Structure	Level	Pattern	Image	Note
TS21: rib pre-cartilage condensation	Strong		9E/F	Intense expression in mesenchymal cells that morphologically resemble chondrocytes.

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Strong		9G/H	Intense expression in mesenchymal cells that morphologically resemble chondrocytes.

Structure	Level	Pattern	Image	Note
TS23: rib	Strong	Regionally restricted	9J/K	Intense expression in mesenchymal cells that morphologically resemble chondrocytes.
TS23: thoracic vertebra	Strong	Regionally restricted	9J/K	Intense expression in mesenchymal cells that morphologically resemble chondrocytes.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	9L/M	Expression begins in the middle of condensing mesenchymal cells that form the humerus, ulna and radius.
TS20: humerus pre-cartilage condensation	Present		9L/M	Expression begins in the middle of condensing mesenchymal cells that form the humerus, ulna and radius.
TS20: radius-ulna pre-cartilage condensation	Present		9L/M	Expression begins in the middle of condensing mesenchymal cells that form the humerus, ulna and radius.

Structure	Level	Pattern	Image	Note
TS23: forelimb pre-cartilage condensation	Present	Regionally restricted	9N/O	Expression restricted to chondrocytes adjacent to hypertrophic chondrocytes and to the perichondrium.