Probe:	pab9SSE
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS21: inferior cervical ganglion	Strong		6_PHOX-2 top	Strong expression in the superior cervical and stellate ganglia.
TS21: superior cervical ganglion	Strong		6_PHOX-2 top	Strong expression in the superior cervical and stellate ganglia.

Structure	Level	Pattern	Image	Note
TS21: thoracic ganglion	Strong		6_PHOX-2 center	Strong expression in the thoracic sympathetic chain.
TS21: sympathetic ganglion	Strong	Regionally restricted	6_PHOX-2 center	Strong expression in the preaortic sympathetic ganglia.

Structure	Level	Pattern	Image	Note
TS21: gland	Present	Regionally restricted	6_PHOX-2 bottom	Slightly weaker expression in the adrenal medulla.

Structure	Level	Pattern	Image	Note
TS21: superior cervical ganglion	Present		7B
TS21: parasympathetic nervous system	Present	Regionally restricted	7B	Expression in all parasympathetic ganglia, of which the sphenopalatine and submandibular ganglia are shown.
TS21: inferior vagus ganglion	Weak		7B	Faint expression in the nodose (X) ganglion.
TS21: vagus ganglion	Weak	Regionally restricted	7B	No expression in the proximal Xth ganglion, which is of neural crest origin
TS21: trigeminal ganglion	Absent		7B	No expression in the trigeminal (V) ganglion.

Structure	Level	Pattern	Image	Note
TS21: enteric nervous system	Present	Regionally restricted	7D	Expression in enteric ganglia (in the wall of the stomach).

Structure	Level	Pattern	Image	Note
TS16: peripheral nervous system	Present	Regionally restricted	8A/B	Expression in paraaortic clusters of cells, ie. the sympathetic ganglion primordium.

Structure	Level	Pattern	Image	Note
TS14: central nervous system	Present	Regionally restricted		Expression in a group of cells adjacent to the otic pit that could correspond to the anlage of the VIIIth ganglion.
TS14: future hindbrain	Weak	Regionally restricted		Faint expression in a few cells in the ventrolateral hindbrain that correspond to the very first postmitotic neurons.
TS14: future spinal cord	Absent			No expression at the level of the future spinal cord.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Absent			No expression in the VIIIth ganglion

Structure	Level	Pattern	Image	Note
TS21: glossopharyngeal ganglion	Weak	Regionally restricted		Weak expression in the petrose (IXth distal) ganglion No expression in the proximal IXth ganglion, which is of neural crest origin.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	9B	In the neural tube, expression restricted to the myelencephalon and isthmic region.
TS17: midbrain	Present	Regionally restricted	9B	In the neural tube, expression restricted to the myelencephalon and isthmic region.
TS17: midbrain-hindbrain junction	Absent		9B	Expression is interrupted at the mes-metencephalic border.
TS17: 4th branchial arch	Strong		9B	Strong expression in the 4th branchial arch which most likely correponds to the area of migration of cells from the nodose placode to the primordium of the nodose ganglion

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	10A/C	In the neural tube, expression restricted to the myelencephalon and isthmic region.
TS17: midbrain	Present	Regionally restricted	10A/C	In the neural tube, expression restricted to the myelencephalon and isthmic region.
TS17: midbrain-hindbrain junction	Absent		10A/C	Expression is interrupted at the mes-metencephalic border.
TS17: rhombomere 4	Present		10A/C	Region of expression extending from rhombomere 4 to 7, to the caudal border of the myelencephalon.
TS17: rhombomere 5	Present		10A/C	Region of expression extending from rhombomere 4 to 7, to the caudal border of the myelencephalon.
TS17: rhombomere 6	Present		10A/C	Region of expression extending from rhombomere 4 to 7, to the caudal border of the myelencephalon.
TS17: rhombomere 7	Present		10A/C	Region of expression extending from rhombomere 4 to 7, to the caudal border of the myelencephalon.

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Strong		10B/D	Strong expression in rhombomeres 4-7, fading out rostrally and caudally to them
TS17: rhombomere 5	Strong		10B/D	Strong expression in rhombomeres 4-7, fading out rostrally and caudally to them
TS17: rhombomere 6	Strong		10B/D	Strong expression in rhombomeres 4-7, fading out rostrally and caudally to them
TS17: rhombomere 7	Strong		10B/D	Strong expression in rhombomeres 4-7, fading out rostrally and caudally to them
TS17: hindbrain	Present	Regionally restricted	10B/D	Strong expression in rhombomeres 4-7, fading out rostrally and caudally to them

Structure	Level	Pattern	Image	Note
TS17: hindbrain lateral wall	Present	Regionally restricted	11B	Expression in a ventral column of cells on each side of the floor and in a dorsolateral column in the alar plate.
TS17: midbrain-hindbrain junction	Present	Regionally restricted	11B	Faint ring of expression at the met-mesencephalic border, interrupted on the dorsal midline and bounded ventrally by two patches of hybridizing cells.
TS17: sympathetic nervous system	Strong	Regionally restricted	11B	Strong expression over the area of the anlage of the nodose ganglion.
TS17: trigeminal ganglion	Absent		11B	No expression in the trigeminal ganglion.

Structure	Level	Pattern	Image	Note
TS17: midbrain lateral wall	Present	Regionally restricted	11D	Expression in a ventral column of cells on each side of the floor plate, plus a dorsolateral column in the alar plate.
TS17: rhombomere 4 lateral wall	Present	Regionally restricted	11D	Expression in a ventral column of cells on each side of the floor plate, plus a dorsolateral column in the alar plate.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	12B	Expression in a restricted set of territories in the hindbrain, corresponding in number, extension and position to noradrenergic and adrenergic nuclei of the hindbrain: areas A1 through 5.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	12C	Expression in a restricted set of territories in the hindbrain, corresponding in number, extension and position to noradrenergic and adrenergic nuclei of the hindbrain: areas A1 through 5.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	12D	Expression in a restricted set of territories in the hindbrain, corresponding in number, extension and position to noradrenergic and adrenergic nuclei of the hindbrain: areas A1 through 5.

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Present	Regionally restricted		Expression in areas C1 and C2 and the area postrema.

Structure	Level	Pattern	Image	Note
TS21: metencephalon	Strong	Regionally restricted	13B	Strong expression in the immature locus coeruleus (area A6).