Probe:	Col4a1 probe5
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Absent		4A	Expression in mesenchymal cells (primitive meninges) surrounding the spinal cord and in blood vessels within the spinal cord, but not in neural tissue.
TS20: embryo mesenchyme	Present	Regionally restricted	4A	Expression in mesenchymal cells (primitive meninges) surrounding the spinal cord and in blood vessels within the spinal cord, but not in neural tissue.
TS20: blood vessel	Present	Regionally restricted	4A	Expression in mesenchymal cells (primitive meninges) surrounding the spinal cord and in blood vessels within the spinal cord, but not in neural tissue.
TS20: tail myocoele	Trace		4A	Expression was either very low or not detected.
TS20: vertebral cartilage condensation	Absent		4A	No expression in neural arches.

Structure	Level	Pattern	Image	Note
TS20: embryo mesenchyme	Strong	Regionally restricted	4B	High levels of expression in mesenchyme cells surrounding ganglia.
TS20: dorsal root ganglion	Present	Regionally restricted	4B	Expression in a subset of cells within the ganglia.
TS20: spinal nerve	Strong		4B	High levels of expression in ganglia fibers and associated nerve roots.
TS20: tail myocoele	Trace		4B	Expression was either very low or not detected.
TS20: vertebral cartilage condensation	Absent		4B	No expression in neural arches.

Structure	Level	Pattern	Image	Note
TS20: cranial nerve	Present	Regionally restricted	4C	High levels of expression in nerve outgrowths in ganglia of the hindbrain, but not within adjacent cells of the hindbrain.
TS20: hindbrain	Absent		4C	High levels of expression in nerve outgrowths in ganglia of the hindbrain, but not within adjacent cells of the hindbrain.

Structure	Level	Pattern	Image	Note
TS20: tongue	Strong	Regionally restricted	10A	Punctate distribution of expression in the tongue and lower jaw, corresponding to developing blood vessels.
TS20: mandible primordium	Present	Regionally restricted	10A	Punctate distribution of expression in the tongue and lower jaw, corresponding to developing blood vessels.

Structure	Level	Pattern	Image	Note
TS20: gut mesenchyme	Strong	Regionally restricted	10B	High levels of expresssion in groups of cells in the mesenchyme of the gut, interspersed between mesenchymal cells with lower levels of expression.
TS20: gut epithelium	Present	Regionally restricted	10B	Expression in epithelial cells of some parts of the gut, but not in other parts.
TS20: dorsal aorta	Strong	Regionally restricted	10B	High levels of expression in mesenchymal cells forming the walls of the aorta.

Structure	Level	Pattern	Image	Note
TS20: stomach mesenchyme	Strong	Regionally restricted	10C	High levels of expresssion in groups of cells in the mesenchyme of the stomach, interspersed between mesenchymal cells with lower levels of expression.
TS20: stomach epithelium	Present		10C	Expression in epithelial cells of the stomach.
TS20: liver	Strong	Regionally restricted	10C	Punctate distribution of expression in the liver, corresponding to developing blood vessels.

Structure	Level	Pattern	Image	Note
TS20: mesenchyme of metanephros	Strong	Regionally restricted	10D	High levels of expresssion in groups of cells in the mesenchyme of the kidney, interspersed between mesenchymal cells with lower levels of expression.
TS20: metanephros	Present	Regionally restricted	10D	Expression in epithelial cells of the kidney.

Structure	Level	Pattern	Image	Note
TS20: heart ventricle	Present	Regionally restricted	10E	Punctate distribution of expression in the ventricle of the heart, corresponding to developing blood vessels.