Probe:	clone 6c
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Absent		2D	No expression in neural tissue.
TS20: embryo mesenchyme	Present	Regionally restricted	2D	Expression in mesenchyme cells surrounding the central nervous system destined to become the meninges.
TS20: tail myocoele	Trace		2D	Expression was either very low or not detected.

Structure	Level	Pattern	Image	Note
TS20: blood vessel	Strong	Regionally restricted	2H	High levels of expression in endothelial cells of blood vessels within the central nervous system.

Structure	Level	Pattern	Image	Note
TS20: embryo mesenchyme	Strong	Regionally restricted	5D	High levels of expression in mesenchyme cells surrounding ganglia.
TS20: dorsal root ganglion	Present	Regionally restricted	5D	Expression in a subset of cells within the ganglia.
TS20: spinal nerve	Strong		5D	High levels of expression in ganglia fibers and associated nerve roots. Expression in dorsal and ventral nerve roots.
TS20: vertebral cartilage condensation	Absent		5D	No expression in neural arches.
TS20: tail myocoele	Trace		5D	Expression was either very low or not detected.

Structure	Level	Pattern	Image	Note
TS20: oral region	Absent		6D
TS20: tongue	Strong	Regionally restricted	6D	Localized to ectodermal cells forming the outer layer of the tongue.

Structure	Level	Pattern	Image	Note
TS20: endocardial lining	Present		7D	Expression restricted to endothelial cells.
TS20: liver	Present		7D

Structure	Level	Pattern	Image	Note
TS20: stomach mesenchyme	Present		8D	Expression restricted to mesenchymal cells.
TS20: stomach epithelium	Absent		8D
TS20: liver	Strong	Regionally restricted	8D	Strong expression in groups of cells in the liver.

Structure	Level	Pattern	Image	Note
TS20: mesenchyme of metanephros	Present		9D	Expression restricted to underlying mesenchymal layer.
TS20: metanephros	Present	Regionally restricted	9D	No expression in epithelial cells.
TS20: dorsal aorta	Strong	Regionally restricted	9D	High levels of expression in mesenchymal cells forming the walls of the aorta.