Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:14429
Thomas T, et al., Genes coding for basement membrane glycoproteins laminin, nidogen, and collagen IV are differentially expressed in the nervous system and by epithelial, endothelial, and mesenchymal cells of the mouse embryo. Exp Cell Res. 1993 Sep;208(1):54-67
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:3845871
|
Gene symbol:
|
Lamc1
|
Gene name:
|
laminin, gamma 1
|
Probe:
|
laminin B2 probe1
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
2C:
embryonic day 12.5
(more )
Specimen
2C:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord ventricular layer |
Strong |
Regionally restricted |
2C
|
High levels of expression in the ventral portion of the ependymal zone cells.
|
TS20: embryo mesenchyme |
Present |
Regionally restricted |
2C
|
Expression in mesenchyme cells surrounding the central nervous system destined to become the meninges.
|
TS20: tail myocoele |
Trace |
|
2C
|
Expression was either very low or not detected.
|
Specimen
2G:
embryonic day 12.5
(more )
Specimen
2G:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: blood vessel |
Weak |
Regionally restricted |
2G
|
Expression in differentiating capillaries
|
Specimen
3B:
embryonic day 12.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5C:
embryonic day 12.5
(more )
Specimen
5C:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6C:
embryonic day 12.5
(more )
Specimen
6C:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: oral region |
Absent |
|
6C
|
|
TS20: tongue |
Weak |
Regionally restricted |
6C
|
Preferentially expressed in isolated groups of cells in the base of the tongue.
|
Specimen
7C:
embryonic day 12.5
(more )
Specimen
7C:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
8C:
embryonic day 12.5
(more )
Specimen
8C:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: stomach epithelium |
Strong |
|
8C
|
Uniform high expression in epithelial cells and surrounding mesenchyme.
|
TS20: stomach mesenchyme |
Strong |
|
8C
|
Uniform high expression in epithelial cells and surrounding mesenchyme.
|
TS20: liver |
Present |
Regionally restricted |
8C
|
Lower levels of expression in groups of cells in the epithelium and througout the mesenchyme.
|
Specimen
9C:
embryonic day 12.5
(more )
Specimen
9C:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: metanephros |
Present |
Uniform |
9C
|
Uniform expression in both epithelial and mesenchymal compartments.
|
TS20: dorsal aorta |
Present |
|
9C
|
Expression in the aorta, essentially the same as in surrounding mesenchyme.
|
|