Probe:	Lama cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Strong		2A	High levels of expresssion in the ependymal layer.
TS20: embryo mesenchyme	Weak	Regionally restricted	2A	Low levels of expression in mesenchymal cells surrounding the central nervous system destined to become the meninges.
TS20: tail myocoele	Ambiguous		2A	Expression was either very low or not detected.

Structure	Level	Pattern	Image	Note
TS20: blood vessel	Absent		2E	No expression in endothelial cells of blood vessels within the central nervous system.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Regionally restricted	3A
TS20: embryo mesenchyme	Present	Regionally restricted	3A
TS20: spinal cord mantle layer	Absent		3A
TS20: vertebral cartilage condensation	Absent		3A	No expression in neural arches.

Structure	Level	Pattern	Image	Note
TS20: hindbrain ventricular layer	Present	Regionally restricted		Expression in the ependymal zone cells of the hindbrain at slightly lower levels than in the spinal cord.
TS20: 4th ventricle choroid plexus	Absent			No expression in the neurectoderm component of the choroid plexus.
TS20: forebrain ventricular layer	Weak			Much lower expression in the ependyma than in the hindbrain.
TS20: midbrain ventricular layer	Weak			Much lower expression in the ependyma than in the hindbrain.
TS20: midbrain	Present	Regionally restricted		Within the midbrain and forebrain, highest levels of expression in the midbrain flexure and in the floor of the diencephalon.
TS20: diencephalon floor plate	Present			Within the midbrain and forebrain, highest levels of expression in the midbrain flexure and in the floor of the diencephalon.

Structure	Level	Pattern	Image	Note
TS20: embryo mesenchyme	Weak	Regionally restricted	5A	Lower levels of expression in mesenchyme cells surrounding ganglia.
TS20: dorsal root ganglion	Absent		5A
TS20: spinal nerve	Absent		5A	No expression in ganglia fibers and associated nerve roots.
TS20: tail myocoele	Ambiguous		5A	Expression was either very low or not detected.

Structure	Level	Pattern	Image	Note
TS20: oral region	Absent		6A
TS20: tongue	Weak		6A	Localized to ectodermal cells forming the outer layer of the tongue.

Structure	Level	Pattern	Image	Note
TS20: heart	Absent		7A
TS20: liver	Absent		7A

Structure	Level	Pattern	Image	Note
TS20: stomach epithelium	Weak		8A
TS20: stomach mesenchyme	Weak		8A
TS20: liver	Absent		8A

Structure	Level	Pattern	Image	Note
TS20: metanephros	Strong	Regionally restricted	9A	High levels of expression in the epithelial buds.
TS20: mesenchyme of metanephros	Absent		9A
TS20: dorsal aorta	Absent		9A