Probe:	Gbx2 probe8
Probe preparation:	labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	1i	Expression in the prospective dorsal thalamus (DT). Caudal boundary of expression abuts on the pretectum (alar p1); rostral boundary at the zona limitans.
TS20: hindbrain	Present	Regionally restricted	1i	In the hindbrain, rostral end of expression domain in the region derived from rhombomere r6.
TS20: alar columns	Present	Uniform	1i	Uniform expression in both alar and basal plates
TS20: basal columns	Present	Uniform	1i	Uniform expression in both alar and basal plates
TS20: spinal cord	Weak	Regionally restricted	1i	Much less or no expression in a thin logitudinal band in the intermediate zone.
TS20: spinal cord floor plate	Absent		1i
TS20: spinal cord roof plate	Absent		1i

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	1j	Expression in the prospective dorsal thalamus. Caudal boundary of expression abuts on the pretectum (alar p1); rostral boundary at the zona limitans).
TS20: epithalamus	Absent		1j	No expression ventrally to the alar/basal boundary or in the epithalamus.
TS20: hindbrain	Present	Regionally restricted	1j	In the hindbrain, rostral end of expression domain in the region derived from rhombomere r6.
TS20: alar columns	Present	Uniform	1j	Uniform expression in both alar and basal plates
TS20: basal columns	Present	Uniform	1j	Uniform expression in both alar and basal plates
TS20: spinal cord	Weak	Regionally restricted	1j	Much less or no expression in a thin logitudinal band in the intermediate zone.
TS20: spinal cord floor plate	Absent		1j
TS20: spinal cord roof plate	Absent		1j

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	1k	Expression in the prospective dorsal thalamus. Caudal boundary of expression abuts on the pretectum (alar p1); rostral boundary at the zona limitans.
TS20: epithalamus	Absent		1k	No expression ventrally to the alar/basal boundary or in the epithalamus.
TS20: hindbrain	Present	Regionally restricted	1k	In the hindbrain, rostral end of expression domain in the region derived from rhombomere r6.
TS20: alar columns	Present	Uniform	1k	Uniform expression in both alar and basal plates
TS20: basal columns	Present	Uniform	1k	Uniform expression in both alar and basal plates
TS20: spinal cord	Weak	Regionally restricted	1k	Much less or no expression in a thin logitudinal band in the intermediate zone.
TS20: spinal cord floor plate	Absent		1k
TS20: spinal cord roof plate	Absent		1k

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1l	Expression in the basal telencephalon and anterior entopeduncular area (AEP).
TS20: thalamus	Present	Regionally restricted	1l	Expression in the prospective dorsal thalamus. Caudal boundary of expression abuts on the pretectum (alar p1); rostral boundary at the zona limitans.
TS20: hindbrain	Present	Regionally restricted	1l	In the hindbrain, rostral end of expression domain in the region derived from rhombomere r6.
TS20: alar columns	Present	Uniform	1l	Uniform expression in both alar and basal plates.
TS20: basal columns	Present	Uniform	1l	Uniform expression in both alar and basal plates.
TS20: spinal cord	Weak	Regionally restricted	1l	Much less or no expression in a thin logitudinal band in the intermediate zone.
TS20: spinal cord floor plate	Absent		1l
TS20: spinal cord roof plate	Absent		1l
TS20: medial ganglionic eminence	Present	Regionally restricted	1l	Expression is in cells centered in the mantle of the rostralmost part of the medial ganglionic eminence (MGE).

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	2g	Expression in the prospective dorsal thalamus. Caudal boundary of expression abuts on the pretectum (alar p1); rostral boundary at the zona limitans.
TS20: epithalamus	Absent		2g	No expression ventrally to the alar/basal boundary or in the epithalamus.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	2h	Expression primarily in cells in the subventricular and mantle layers within the wall of the diencephalon.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2i	Expression in the basal telencephalon and anterior entopeduncular area (AEP).
TS20: medial ganglionic eminence	Present	Regionally restricted	2i	Expression is in cells centered in the mantle of the rostralmost part of the medial ganglionic eminence (MGE).

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	3c	Expression in the prospective dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3d	Expression in the anterior entopeduncular (AEP).
TS20: medial ganglionic eminence	Present	Regionally restricted	3d	Expression in the mantle of the rostralmost part of the median ganglionic eminence (MGE).

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	4a	Expression in the prospective dorsal thalamus.
TS20: epithalamus	Absent		4a	No expression ventrally to the alar/basal boundary or in the epithalamus.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	4b	Expression primarily in cells in the subventricular and mantle layers within the wall of the diencephalon, defining 4 zones in the dorsal diencephalon wall.