Probe:	Dlx1 cDNA2
Probe preparation:	labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1a	Expression domain includes the septum (SE).
TS20: cerebral cortex	Absent		1a	No expression in the entire neuroepithelium of the cerebral cortex.
TS20: diencephalon	Present	Regionally restricted	1a	Second expression domain includes the hypothalamic cell cord (HCC), posterior entopeduncular area (PEP) and ventral thalamus (VT). No expression in the mammillary area (MA).
TS20: hypothalamus	Present	Regionally restricted	1a	Second expression domain includes the hypothalamic cell cord (HCC), posterior entopeduncular area (PEP) and ventral thalamus (VT). No expression in the mammillary area (MA).
TS20: thalamus	Present	Regionally restricted	1a	Second expression domain includes the hypothalamic cell cord (HCC), posterior entopeduncular area (PEP) and ventral thalamus (VT). No expression in the mammillary area (MA).
TS20: medial ganglionic eminence	Present		1a

Structure	Level	Pattern	Image	Note
TS20: diencephalon lamina terminalis	Present	Regionally restricted	1b	Expression domain includes the septum (SE). Medially, expression domain is continuous with the lamina terminalis.
TS20: telencephalon	Present	Regionally restricted	1b	Expression domain includes the septum (SE). Medially, expression domain is continuous with the lamina terminalis.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1c	Expression domain includes the septum (SE).
TS20: diencephalon	Present	Regionally restricted	1c	Second expression domain includes the suprachiasmic area (SCH), hypothalamic cell cord (HCC) and ventral thalamus (VT). No expression in the mammillary area (MA.
TS20: hypothalamus	Present	Regionally restricted	1c	Second expression domain includes the suprachiasmic area (SCH), hypothalamic cell cord (HCC) and ventral thalamus (VT). No expression in the mammillary area (MA.
TS20: thalamus	Present	Regionally restricted	1c	Second expression domain includes the suprachiasmic area (SCH), hypothalamic cell cord (HCC) and ventral thalamus (VT). No expression in the mammillary area (MA.
TS20: lateral ganglionic eminence	Present		1c
TS20: medial ganglionic eminence	Present		1c

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1d	Expression domain includes the medial ganglionic eminence (MGE), No expression in the supraoptic/paraventricular area (SPV), the eminentia thalami (EMT) and posterior preoptic area (POP).
TS20: diencephalon	Present	Regionally restricted	1d	Second expression domain includes the suprachiasmic area (SCH), posterior entopeduncular area (PEP) and ventral thalamus (VT). Weak expression in lateral parts of the tuberal hypothalamus (TU).
TS20: thalamus	Present	Regionally restricted	1d	Second expression domain includes the suprachiasmic area (SCH), posterior entopeduncular area (PEP) and ventral thalamus (VT). Weak expression in lateral parts of the tuberal hypothalamus (TU).
TS20: medial ganglionic eminence	Present		1d

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Present		2a
TS20: diencephalon	Present	Regionally restricted	2a	Second expression domain in the hypothalamic cell cord (HCC), posterior entopeduncular area (PEP) and ventral thalamus (VT).
TS20: hypothalamus	Present	Regionally restricted	2a	Second expression domain in the hypothalamic cell cord (HCC), posterior entopeduncular area (PEP) and ventral thalamus (VT).
TS20: thalamus	Present	Regionally restricted	2a	Second expression domain in the hypothalamic cell cord (HCC), posterior entopeduncular area (PEP) and ventral thalamus (VT).

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2b	Expression domain includes the medial ganglionic eminence (MGE) and lateral ganglionic eminence LGE). No expression in the supraoptic/paraventricular area (SPV) and the eminentia thalami (EMT).
TS20: hypothalamus	Present	Regionally restricted	2b	Second expression domain in the hypothalamic cell cord (HCC).
TS20: lateral ganglionic eminence	Present		2b
TS20: medial ganglionic eminence	Present		2b

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2c	Expression domain includes the medial ganglionic eminence (MGE), lateral ganglionic eminence LGE), and the septum (SE). No expression in the posterior preoptic area (POP).
TS20: diencephalon	Present	Regionally restricted	2c	Second expression domain in the suprachiasmic area (SCH).
TS20: lateral ganglionic eminence	Present		2c
TS20: medial ganglionic eminence	Present		2c