Cux1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:11951 Andres V, et al., Clox, a mammalian homeobox gene related to Drosophila cut, encodes DNA-binding regulatory proteins differentially expressed during development. Development. 1992 Oct;116(2):321-34
Assay type:	Western blot
MGI Accession ID:	MGI:3845534
Gene symbol:	Cux1
Gene name:	cut-like homeobox 1

Antibody:	anti-FP
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Authors report that the patterns of expression were similar to that seen with the anti-P antibody. Results were not shown.

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	250 kDa (b)	190 kDa (b)	Genetic Background	Mutant Allele(s)	Sex	Note
Lane 1	E15.0 (a)	TS23: axial skeleton	Present	Present	CD-1		Not Specified	vertebral column and ribs
Lane 2	E15.0 (a)	TS23: liver	Present	Present	CD-1		Not Specified
Lane 3	E15.0 (a)	TS23: brain	Present	Present	CD-1		Not Specified
Lane 4	E15.0 (a)	TS23: lung	Present	Present	CD-1		Not Specified
Lane 5	E15.0 (a)	TS23: heart	Present	Present	CD-1		Not Specified
Lane 6	E15.0 (a)	TS23: vertebral axis musculature	Present	Present	CD-1		Not Specified
Lane 7	P m 1	TS28: xiphisternum	Trace (c)	Trace (c)	CD-1		Not Specified	xiphoid process
Lane 8	P m 1	TS28: liver	Trace (c)	Trace (c)	CD-1		Not Specified
Lane 9	P m 1	TS28: brain	Present (d)	Present (e)	CD-1		Not Specified
Lane 10	P m 1	TS28: lung	Present (d)	Present (e)	CD-1		Not Specified
Lane 11	P m 1	TS28: heart	Present (d)	Present (e)	CD-1		Not Specified
Lane 12	P m 1	TS28: skeletal muscle	Trace (c)	Trace (c)	CD-1		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Bands were arbitrarily categorized into two groups: ~180-190 kDa, which predominate in early embryogenesis; and ~230-250 kDa, which are the predominant forms in the adult. Faint bands below 180 kDa were not considered specific.
(c) Bands were barely detectable.
(d) Increase in 230-250 kDa bands; predominant species in these tissues.
(e) Decrease in 180-190 kDa bands.

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