Gene Expression Data
Assay Details
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Assay
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Reference:
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J:138310
Xu C, et al., Expression of Robo/Slit and Semaphorin/Plexin/Neuropilin family members in the developing hypothalamic paraventricular and supraoptic nuclei. Gene Expr Patterns. 2008 Sep;8(7-8):502-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3845303
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Gene symbol:
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Sema5b
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Gene name:
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sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B
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Probe:
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Sema5b cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Fixative was Methancarn (60% methanol,
30% chloroform, and 10% acetic acid).
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Results
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Specimen
3C1:
embryonic day 12.5
(more  )
Specimen
3C1:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
3_C1
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Expression is in the ventricular lining.
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| TS20: hypothalamus |
Present |
Regionally restricted |
3_C1
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Expression is in a domain just below the progenitor of the paraventricular nucleus/supraoptic nucleus.
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Specimen
3C2, C5:
embryonic day 13.5
(more )
Note: (C5) is a higher magnification of the boxed area in (C2).
Specimen
3C2, C5:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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(C5) is a higher magnification of the boxed area in (C2).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: diencephalon |
Present |
Regionally restricted |
3_C2
,
3_C5
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Expression is in cells lining the third ventricle.
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Specimen
3C3:
embryonic day 14.5
(more )
Specimen
3C3:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
3_C3
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Expression is in cells lining the third ventricle.
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Specimen
3C4:
embryonic day 15.5
(more )
Specimen
3C4:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: diencephalon |
Present |
Regionally restricted |
3_C4
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Expression is in cells lining the third ventricle.
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| TS23: hypothalamus |
Weak |
Regionally restricted |
3_C4
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Expression is in the medial paraventricular nucleus.
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Analysis Tools