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Gene Expression Data
Assay Details
Assay
Reference: J:12566 O'Brien TX, et al., Positional specification of ventricular myosin light chain 2 expression in the primitive murine heart tube. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5157-61
Assay type: RNA in situ
MGI Accession ID: MGI:3845039
Gene symbol: Myl2
Gene name: myosin, light polypeptide 2, regulatory, cardiac, slow
Probe: MLC-2v probe2
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen not shown: embryonic day 7.0 (more )
Structure Level Pattern Image Note
TS10: embryo Absent Multiple embryos examined at day 7 pc (prior to heart tube formation) showed no expression above background.
Specimen 1A,B: embryonic day 8.0 (more )
Structure Level Pattern Image Note
TS12: primitive ventricle Strong 1A/B
TS12: heart Present Regionally restricted 1A/B Authors report no levels above background elsewhere in the developing heart.
Specimen 1C,D: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: primitive ventricle Strong 1C/D
TS13: outflow tract Present Regionally restricted 1C/D Less intense expression in the proximal outflow tract.
TS13: common atrial chamber Absent 1C/D
TS13: sinus venosus Absent 1C/D
Specimen 1E,F: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: primitive ventricle Strong 1E/F Strong expression in the ventricular region, with a clear demarcation at the junction with the atrial region.
TS14: common atrial chamber Absent 1E/F
Specimen 1G,H: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: primitive ventricle Strong 1G/H
TS14: outflow tract Strong Regionally restricted 1G/H Expression in the proximal outflow tract immediately adjacent to ventricular region.
Specimen 1I,J: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: outflow tract Present 1I/J
TS14: primitive ventricle Present 1I/J
Specimen 2A,B: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: primitive ventricle cardiac muscle Strong 2A/B
TS16: outflow tract Strong Regionally restricted 2A/B Intense expression further into the outflow tract than previously. No expression in outflow tract cushion tissues.
Specimen 2C,D: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: primitive ventricle Present Regionally restricted 2C/D Expression in the superior aspect of the ventricular septum.
TS18: primitive ventricle cardiac muscle Weak Regionally restricted 2C/D Barely detectable expression in the myocardium adjacent to the outflow tract cushions.
TS18: outflow tract Absent 2C/D No detectable expression in the proximal outflow tract.
Specimen 2E,F: embryonic day 15.0 (more )
Structure Level Pattern Image Note
TS23: heart ventricle Present 2E/F
Specimen 2H,I: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: heart ventricle Present 2H/I
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory