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Tg(CAG-luc,-GFP)L2G85Chco
Transgene Detail
Summary
Symbol: Tg(CAG-luc,-GFP)L2G85Chco
Name: transgene insertion L2G85, Christopher H Contag
MGI ID: MGI:3844218
Synonyms: L2685, L2G85, luc+, Tg (CAG- Luc, GFP)
Transgene: Tg(CAG-luc,-GFP)L2G85Chco  Location: unknown  Genetic Position: Chr7, Syntenic
Alliance: Tg(CAG-luc,-GFP)L2G85Chco page
Transgene
origin
Strain of Origin:  FVB
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
  Tg(CAG-luc,-GFP)L2G85Chco involves 1 genes/genome features (Ptprh) View all
 
Mutation details: A transgenic construct containing a modified firefly luciferase gene open reading frame, 54bp of the foot and mouth disease virus (FMDV) 2A sequence (ribsome slippage site) and eGFP sequence under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer (CAG), was injected into fertilized FVB mouse eggs. Founder line L2G85 animals were bred to wildtype FVB mice. (J:137739, J:148234)
Tumor Data
List all tumor models in MMHCdb carrying Tg(CAG-luc,-GFP)L2G85Chco
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 6 strains available      Cell Lines: 0 lines available
Notes
Fine mapping at The Jackson Laboratory indicates that the transgene inserted into Ptprh exon 10 [Chr7:4,558,956-4,569,937 ; mm10]. Luciferase ddPCR performed on homozygous mice (n=17) showed ~11-19 copies of the transgene. Western blot of intestinal tissue from homozygous mice (n=6) showed no PTPRH expression.
References
Original:  J:148234 Cao YA, et al., Shifting foci of hematopoiesis during reconstitution from single stem cells. Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):221-6
All:  48 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory