Gene Expression Data
Assay Details
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Assay
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Reference:
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J:85036
Goncalves-Mendes N, et al., Mouse SCO-spondin, a gene of the thrombospondin type 1 repeat (TSR) superfamily expressed in the brain. Gene. 2003 Jul 17;312:263-70
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3844059
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Gene symbol:
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Sspo
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Gene name:
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SCO-spondin
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Probe:
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lambda ZAPC5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Alkaline hydrolysis was performed to reduce the probe
length to 300-350 nucleotides. Fixative was 4% formaldehyde.
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Results
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Specimen
3C:
postnatal adult
(more  )
Specimen
3C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: diencephalon |
Very strong |
Regionally restricted |
3C
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Expression is in the the secretory ependymal cells of the subcommissural organ.
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| TS28: midbrain |
Very strong |
Regionally restricted |
3C
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Expression is in the the secretory ependymal cells of the subcommissural organ.
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Specimen
3D:
embryonic day 15.0
(more )
Specimen
3D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: diencephalon |
Weak |
Regionally restricted |
3D
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Expression is in the anlage of the subcommissural organ.
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| TS23: midbrain |
Weak |
Regionally restricted |
3D
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Expression is in the anlage of the subcommissural organ.
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Specimen
3E:
embryonic day 17.0
(more )
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: diencephalon |
Present |
Regionally restricted |
3E
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Expression is in the subcommissural organ.
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| TS25: midbrain |
Present |
Regionally restricted |
3E
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Expression is in the subcommissural organ.
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Specimen
3F:
postnatal day 1
(more )
Specimen
3F:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: diencephalon |
Strong |
Regionally restricted |
3F
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Expression is in the subcommissural organ.
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| TS27: midbrain |
Strong |
Regionally restricted |
3F
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Expression is in the subcommissural organ.
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Specimen
3G:
postnatal adult
(more )
Specimen
3G:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: diencephalon |
Very strong |
Regionally restricted |
3G
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Expression is in the the secretory ependymal cells of the subcommissural organ.
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| TS28: midbrain |
Very strong |
Regionally restricted |
3G
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Expression is in the the secretory ependymal cells of the subcommissural organ.
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Analysis Tools