Probe:	int-2f
Probe preparation:	Antisense labelled with S35 RNA
Assay notes:	Authors report identical results with the int-2g (MGI:10698) probe.

Structure	Level	Pattern	Image	Note
TS12: parietal endoderm	Present		2C2 , 2C1
TS12: extraembryonic visceral endoderm	Absent		2C1 , 2C2

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		3_2 , 3_1	Expression in cells migrating through the primitive streak.
TS11: extraembryonic mesoderm	Present	Regionally restricted	3_1 , 3_2	Authors report expression in cells at the posterior end of the embryo destined to form predominantly extraembryonic mesoderm.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		3_5 , 3_6
TS11: extraembryonic mesoderm	Present	Regionally restricted	3_6 , 3_5	Authors report expression in cells moving from the primitive streak to extraembryonic sites.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present		3_7 , 3_8
TS12: extraembryonic mesenchyme	Present	Regionally restricted	3_7 , 3_8	Authors report expression in extraembryonic mesoderm surrounding the entire exocoelomic cavity.

Structure	Level	Pattern	Image	Note
TS15: embryo mesenchyme	Present	Regionally restricted	3_10 , 3_9	Expression in mesodermal cells migrating through the primitive streak.
TS15: somite	Absent		3_10 , 3_9
TS15: tail unsegmented mesenchyme	Absent		3_10 , 3_9

Structure	Level	Pattern	Image	Note
TS15: embryo mesenchyme	Present	Regionally restricted	3_11 , 3_12	Expression in mesodermal cells migrating through the primitive streak.

Structure	Level	Pattern	Image	Note
TS12: parietal endoderm	Present		5_1 , 5_2
TS12: primitive streak	Present		5_1 , 5_2
TS12: future hindbrain	Present	Regionally restricted	5_1 , 5_2	Expression in neuroepithelium of the myelencephalon.
TS12: extraembryonic mesenchyme	Absent		5_2 , 5_1	Authors report expression is no longer present in extraembryonic mesoderm

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	5_4 , 5_3	Expression in neuroepithelium of the myelencephalon, restricted to cells adjacent to developing otocysts.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	5_6 , 5_5	Expression in neuroepithelium of the myelencephalon.
TS15: 1st branchial pouch endoderm	Present	Regionally restricted	5_5 , 5_6	Expression in endoderm of pharyngeal pouches, restricted to the lower surface of the 1st and 2nd pouch.
TS15: 2nd branchial pouch endoderm	Present	Regionally restricted	5_5 , 5_6	Expression in endoderm of pharyngeal pouches, restricted to the lower surface of the 1st and 2nd pouch.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial pouch endoderm	Present	Regionally restricted	5_7 , 5_8	Expression in endoderm of pharyngeal pouches, restricted to the lower surface of the 1st and 2nd pouch.
TS15: 2nd branchial pouch endoderm	Present	Regionally restricted	5_7 , 5_8	Expression in endoderm of pharyngeal pouches, restricted to the lower surface of the 1st and 2nd pouch.