Probe:	Sna probe4
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS9: embryo mesoderm	Present		3A/B
TS9: embryo ectoderm	Present	Regionally restricted	3A/B	Lower levels of expression in primitive ectoderm in the primitive streak, but not in other regions of primitive ectoderm.
TS9: primitive streak	Present		3A/B	Lower levels of expression in primitive ectoderm in the primitive streak, but not in other regions of primitive ectoderm.
TS9: primitive endoderm	Present	Regionally restricted	3A/B	Expression in parietal endoderm, derived from primitive endoderm.

Structure	Level	Pattern	Image	Note
TS9: embryo mesoderm	Present		3C/D

Structure	Level	Pattern	Image	Note
TS9: ectoplacental cone	Present		3E/F
TS9: embryo mesoderm	Present		3E/F
TS9: parietal endoderm	Present		3E/F

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present		3G/H
TS11: primitive streak	Weak		3G/H	Lower levels of expression in primitive ectoderm in the primitive streak.
TS11: ectoplacental cone	Present		3G/H

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present		3I/L
TS11: primitive streak	Weak		3I/L	Lower levels of expression in primitive ectoderm in the primitive streak.
TS11: parietal endoderm	Present		3I/L	Expression in parietal endoderm, derived from primitive endoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present		3J/M
TS11: primitive streak	Weak		3J/M	Lower levels of expression in primitive ectoderm in the primitive streak.
TS11: parietal endoderm	Present		3J/M	Expression in parietal endoderm, derived from primitive endoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present		3K/N
TS11: primitive streak	Weak		3K/N	Lower levels of expression in primitive ectoderm in the primitive streak.
TS11: parietal endoderm	Present		3K/N

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Present	Regionally restricted	4A/B	Expression in trophectoderm and parietal endoderm.
TS13: allantois	Present	Regionally restricted	4A/B	Expression in allantois mesoderm.

Structure	Level	Pattern	Image	Note
TS13: embryo mesenchyme	Present		4C/D	Expression in the mesoderm in the embryo proper.
TS13: primitive streak	Absent		4C/D	No expression in the primitive ectoderm in the primitive streak.

Structure	Level	Pattern	Image	Note
TS13: embryo mesenchyme	Present		4E/F	Expression in the mesoderm in the embryo proper.
TS13: trunk unsegmented mesenchyme	Present		4E/F	Expression in the posterior of the embryo in presomitive mesoderm.
TS13: lateral plate mesoderm	Present		4E/F	Expression in anterior regions of the embryo in paraxial and lateral mesoderm.
TS13: trunk paraxial mesenchyme	Present		4E/F	Expression in anterior regions of the embryo in paraxial and lateral mesoderm.
TS13: neural ectoderm	Present	Regionally restricted	4E/F	Expression at the edges of the neural plate.
TS13: future spinal cord neural crest	Present		4E/F	Expression in presumptive premigratory neural crest.
TS13: migrating neural crest	Present		4E/F

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	4G/H	Expression at the edges of the neural plate.
TS13: future spinal cord neural crest	Present		4G/H	Expression in presumptive premigratory neural crest.
TS13: migrating neural crest	Present		4G/H

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present		5A/B	Expression in migrating neural crest cells as they migrate into the branchial arches.
TS15: branchial arch mesenchyme	Weak	Regionally restricted	5A/B	Lower levels of expression in the center of the branchial arches, correlates with the location of mesoderm destined to form the branchial arch muscles.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present		5C/D

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Regionally restricted	5E/F	Authors report no expression in the primitve streak in caudal regions of the embryo.

Structure	Level	Pattern	Image	Note
TS17: trunk somite	Present		5G/H	Authors report restriction of expression to decondensing somites in anterior regions of the trunk.
TS17: dorsal root ganglion	Absent		5G/H	No expression in spinal ganglia (dorsal root ganglia).

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	5I/J	Authors report expression in a lateral-medial gradient in the branchial arches.

Structure	Level	Pattern	Image	Note
TS17: sclerotome	Present		5K/L
TS17: dermatome	Absent		5K/L

Structure	Level	Pattern	Image	Note
TS17: embryo mesenchyme	Present	Regionally restricted	5M/N	No expression in areas of muscle formation.

Structure	Level	Pattern	Image	Note
TS17: myotome	Present		5O/P
TS17: dermatome	Absent		5O/P

Structure	Level	Pattern	Image	Note
TS17: cranial ganglion	Absent

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present		6A/B
TS20: tail sclerotome	Present		6A/B
TS20: vertebral pre-cartilage condensation	Present		6A/B
TS20: cartilage	Present	Regionally restricted	6A/B	Expression in the facial cartilage.

Structure	Level	Pattern	Image	Note
TS20: tail sclerotome	Present		6C/D

Structure	Level	Pattern	Image	Note
TS20: vertebral pre-cartilage condensation	Present		6E/F

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present		6G/H
TS20: rib pre-cartilage condensation	Present		6G/H
TS20: vertebral pre-cartilage condensation	Present		6G/H

Structure	Level	Pattern	Image	Note
TS20: hindlimb pre-cartilage condensation	Present		6I/J

Structure	Level	Pattern	Image	Note
TS20: gut mesenchyme	Present			Expression in mesenchyme of the gut and kidney.
TS20: mesenchyme of metanephros	Present			Expression in mesenchyme of the gut and kidney.
TS20: skeletal muscle	Absent			No expression in developing muscle.

Structure	Level	Pattern	Image	Note
TS22: hindlimb cartilage condensation	Present	Regionally restricted	6K/L	No expression in developing cartilage, except for distal phalanges of the hindlimbs.