Probe:	Mirn449a LNA probe
Probe preparation:	Antisense labelled with digoxigenin
Assay notes:	LNA (locked nucleic acid) oligonucleotides were used for the probe in this assay.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain roof plate	Present	Regionally restricted	2A	At E9.75, expression specifically in the caudal half of the developing roof plate of the 4th ventricle.

Structure	Level	Pattern	Image	Note
TS17: hindbrain roof plate	Present	Regionally restricted	2B	Expression extends anteriorly to the rostral half of the 4th ventricle roof plate.

Structure	Level	Pattern	Image	Note
TS17: hindbrain roof plate	Present	Regionally restricted	2C
TS17: surface ectoderm	Absent		2C	No expression in the overlying ectodermal cell layer.

Structure	Level	Pattern	Image	Note
TS17: hindbrain roof plate	Present	Regionally restricted	2D	Expression extends anteriorly to the rostral half of the 4th ventricle roof plate.

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Present	Regionally restricted	2E	As the roof plate begins to invaginate, expression becomes progressively restricted along the rostro-caudal axis to the region of the roof plate giving rise to the choroid plexus.

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Present	Regionally restricted	2F	As the roof plate begins to invaginate, expression becomes progressively restricted along the rostro-caudal axis to the region of the roof plate giving rise to the choroid plexus.

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Present	Regionally restricted	2G	Expression in the 4th ventricle choroid plexus specific to the epithelial cell layer.

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Present	Regionally restricted	2H	Expression in the 4th ventricle choroid plexus specific to the epithelial cell layer.

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present	Regionally restricted	2J	Expression in the 4th ventricle choroid plexus specific to the epithelial cell layer.

Structure	Level	Pattern	Image	Note
TS21: lateral ventricle choroid plexus	Present	Regionally restricted	2K	Expression in the choroid plexus epithelia of the lateral ventricles.

Structure	Level	Pattern	Image	Note
TS23: 3rd ventricle choroid plexus	Present	Regionally restricted	2L	Expression in the choroid plexus epithelia of the 3rd ventricle.

Structure	Level	Pattern	Image	Note
TS20: hindbrain roof	Present	Regionally restricted	4A	Authors state that the domain of expression overlaps and falls within that of Lmx1a but outside that of Wnt1.