Mir449a Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:147805 Redshaw N, et al., microRNA-449 is a putative regulator of choroid plexus development and function. Brain Res. 2009 Jan 23;1250:20-6
Assay type:	Northern blot
MGI Accession ID:	MGI:3843213
Gene symbol:	Mir449a
Gene name:	microRNA 449a

Probe:	Mirn449a LNA probe
Probe preparation:	Antisense labelled with digoxigenin
Assay notes:	LNA (locked nucleic acid) oligonucleotides were used for the probe in this assay.

Results

Image: 3

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
cerebral cortex	P adult	TS28: cerebral cortex	Weak (a)	20 µg; total RNA	Not Specified		Not Specified
CP	P adult	TS28: lateral ventricle choroid plexus	Very strong (b)	8 µg; total RNA	Not Specified		Not Specified

Notes:
(a) Authors state that expression may be due to contamination with choroid plexus-derived tissue.
(b) Authors report much stronger expression in choroid plexus-derived RNA despite unequal sample loading,

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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