Probe:	ANF probe5
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	1a/a'	Authors report that of five day-8 embryos, expression was observed in two developing hearts.
TS13: cardiac muscle tissue	Present	Regionally restricted	1a/a'	Expression localized to a subpopulation of myocardial cells.
TS13: endocardial tube	Absent		1a/a'	No expression in cells of the endocardial layer of the primitive heart.

Structure	Level	Pattern	Image	Note
TS15: heart atrium	Strong		2a/a'	Prominent expression in atria and primitive ventricle.
TS15: primitive ventricle	Strong		2a/a'	Prominent expression in atria and primitive ventricle.
TS15: outflow tract	Absent		2a/a'	No expression in the truncus arteriosus.

Structure	Level	Pattern	Image	Note
TS15: heart atrium	Strong		2b/b'
TS15: primitive ventricle	Strong		2b/b'
TS15: bulbus cordis	Weak	Regionally restricted	2b/b'	Only faint expression in the bulbus cordis. No expression in the cells of the bulbus cordis nearest the primitive ventricle.

Structure	Level	Pattern	Image	Note
TS15: sinus venosus	Absent

Structure	Level	Pattern	Image	Note
TS22: left atrium	Present		3	Expression in both atria.
TS22: right atrium	Present		3	Expression in both atria.
TS22: heart left ventricle	Present		3	Expression in the left ventricle comparable to atrial expression.
TS22: heart right ventricle	Weak		3	Markedly fewer expressing cells in the right ventricle.

Structure	Level	Pattern	Image	Note
TS22: arch of aorta	Absent			Authors report no expression in outflow tracts, arch of aorta, cardiac valves, or interventricular septum.
TS22: heart valve	Absent			Authors report no expression in outflow tracts, arch of aorta, cardiac valves, or interventricular septum.
TS22: interventricular septum	Absent			Authors report no expression in outflow tracts, arch of aorta, cardiac valves, or interventricular septum.
TS22: outflow tract aortic component	Absent			Authors report no expression in outflow tracts, arch of aorta, cardiac valves, or interventricular septum.
TS22: outflow tract pulmonary component	Absent			Authors report no expression in outflow tracts, arch of aorta, cardiac valves, or interventricular septum.

Structure	Level	Pattern	Image	Note
TS27: left atrium	Strong		4a	Expression in both atria.
TS27: right atrium	Strong		4a	Expression in both atria.
TS27: heart ventricle	Weak	Regionally restricted	4a	Expression in ventricles at levels considerably lower than in the atria, mainly confined to trabeculated regions.