Gpr12 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:81917 Ignatov A, et al., Role of the G-protein-coupled receptor GPR12 as high-affinity receptor for sphingosylphosphorylcholine and its expression and function in brain development. J Neurosci. 2003 Feb 1;23(3):907-14
Assay type:	RNA in situ
MGI Accession ID:	MGI:3842695
Gene symbol:	Gpr12
Gene name:	G-protein coupled receptor 12

Probe:	Gpr12 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Results

4A E14.5 ©

Specimen 4A E14.5: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Strong		4A E14.5
TS22: cerebral cortex	Very strong		4A E14.5

4A E16.5 ©

Specimen 4A E16.5: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: cerebral cortex	Very strong	4A E16.5
TS24: hippocampus	Very strong	4A E16.5
TS24: brainstem	Present	4A E16.5
TS24: liver	Present	4A E16.5
TS24: spinal cord	Present	4A E16.5

4A E18.5 ©

Specimen 4A E18.5: embryonic day 18.5 (more

)

Structure	Level	Image
TS26: central nervous system	Present	4A E18.5
TS26: intestine	Weak	4A E18.5
TS26: stomach	Weak	4A E18.5

4B ©

Specimen 4B: embryonic day 14.5 (more

)

Structure	Level	Image
TS22: cortical plate	Strong	4B
TS22: piriform cortex	Strong	4B
TS22: caudate nucleus	Present	4B

4C ©

Specimen 4C: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Strong		4C
TS22: caudate nucleus	Present		4C

4D ©

Specimen 4D: embryonic day 14.5 (more

)

Structure	Level	Image
TS22: cortical plate	Strong	4D
TS22: hippocampus	Strong	4D
TS22: caudate nucleus	Present	4D

4E ©

Specimen 4E: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Strong		4E

4F ©

Specimen 4F: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	4F	Expression is in the dorsomedial and arcuate nuclei.

4G ©

Specimen 4G: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4G	Expression is in the mammillary body.

4H ©

Specimen 4H: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: brainstem	Very strong	Regionally restricted	4H	Expression is in the motor and sensor nuclei in the medullary reticular formation.

4I ©

Specimen 4I: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: brainstem	Present	Regionally restricted	4I	Expression is in the motor and sensor nuclei in the medullary reticular formation.

5A ©

Specimen 5A: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Very strong		5A
TS28: main olfactory bulb mitral cell layer	Very strong		5A

5B ©

Specimen 5B: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: accumbens nucleus	Present		5B
TS28: piriform cortex	Present		5B

5C ©

Specimen 5C: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Present		5C
TS28: corpus callosum	Absent		5C

5D ©

Specimen 5D: postnatal adult (more

)

Structure	Level	Image
TS28: piriform cortex	Present	5D
TS28: telencephalon septum	Present	5D
TS28: corpus callosum	Absent	5D

5E ©

Specimen 5E: postnatal adult (more

)

Structure	Level	Image
TS28: piriform cortex	Present	5E
TS28: telencephalon septum	Present	5E
TS28: corpus callosum	Absent	5E

5F ©

Specimen 5F: postnatal adult (more

)

Structure	Level	Image
TS28: hippocampus CA2	Very strong	5F
TS28: CA1 pyramidal cell layer	Strong	5F
TS28: dentate gyrus granule cell layer	Strong	5F
TS28: hippocampus CA3	Strong	5F
TS28: corpus callosum	Absent	5F

5G ©

Specimen 5G: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		5G
TS28: corpus callosum	Absent		5G

5H ©

Specimen 5H: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Strong	Regionally restricted	5H	Expression is in the retrospenial cortex.
TS28: amygdala	Very strong		5H
TS28: geniculate thalamic group	Very strong		5H

5I ©

Specimen 5I: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: hindbrain	Weak		5I
TS28: cerebellum Purkinje cell layer	Weak	Single cells	5I	Expression is in some of the Purkinje cells located in lobules 9 and 10.

5J ©

5K ©

Specimen 5J,K: postnatal adult (more

)
Note: Brightfield/darkfield pair.

Structure	Level	Image	Note
TS28: hippocampus CA2	Very strong	5K , 5J	Expression is in neurons.
TS28: CA1 pyramidal cell layer	Strong	5K , 5J	Expression is in neurons.
TS28: corpus callosum	Absent	5J , 5K

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