Myog RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:142168 Seo KW, Dmrt2 and Pax3 double-knockout mice show severe defects in embryonic myogenesis. Comp Med. 2007 Oct;57(5):460-8
Assay type:	RNA in situ
MGI Accession ID:	MGI:3842331
Gene symbol:	Myog
Gene name:	myogenin

Probe:	pMYOG-2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 2A: embryonic day 11.5; Pax3^Sp/Pax3⁺ (more

)

Structure	Level	Image
TS19: forelimb bud	Present	2A
TS19: hindlimb bud	Present	2A
TS19: trunk somite	Present	2A

Specimen 2B: embryonic day 11.5; Dmrt2^tm1Rjo/Dmrt2⁺, Pax3^Sp/Pax3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Present		2B	Expression within the somites comparable with that in wild-type embryos.

Specimen 2C: embryonic day 11.5; Dmrt2^tm1Rjo/Dmrt2^tm1Rjo (more

)

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Present	Regionally restricted	2C	Abnormal arrangement of expressing cells in interlimb somites; Both limb buds contain expressing cells.
TS19: trunk paraxial mesenchyme	Present	Regionally restricted	2C	Expressing cells present in the intersomite area.
TS19: forelimb bud	Present		2C
TS19: hindlimb bud	Present		2C

Specimen 2D: embryonic day 11.5; Dmrt2^tm1Rjo/Dmrt2^tm1Rjo, Pax3^Sp/Pax3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Present	Regionally restricted	2D	Pattern of expression is similar to that in seen in 2C.

Specimen 2E: embryonic day 11.5; Pax3^Sp/Pax3^Sp (more

)

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Present	Regionally restricted	2E	Altered pattern of expression in somites of the interlimb region.
TS19: trunk paraxial mesenchyme	Present	Regionally restricted	2E	Expressing cells not confined within the somite boundary in the hypaxial myotome.

Specimen 2F: embryonic day 11.5; Dmrt2^tm1Rjo/Dmrt2⁺, Pax3^Sp/Pax3^Sp (more

)

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Present	Regionally restricted	2F	Expressing cells scattered in the hypaxial region.

Specimen 3A: embryonic day 11.5; Dmrt2^tm1Rjo/Dmrt2⁺, Pax3^Sp/Pax3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		3A	Expression in the limbs comparable with that in wild-type embryos.
TS19: hindlimb bud	Present		3A	Expression in the limbs comparable with that in wild-type embryos.

Specimen 3B: embryonic day 11.5; Dmrt2^tm1Rjo/Dmrt2⁺, Pax3^Sp/Pax3^Sp (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Weak	Regionally restricted	3B	Expression in both limbs severely decreased. Authors report abnormal myogenic cell migration in both limbs.
TS19: hindlimb bud	Weak	Regionally restricted	3B	Expression in both limbs severely decreased. Authors report abnormal myogenic cell migration in both limbs.

Specimen 3C: embryonic day 11.5; Dmrt2^tm1Rjo/Dmrt2^tm1Rjo, Pax3^Sp/Pax3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Weak		3C	Authors state that expression in the forelimb bud markedly reduced.
TS19: hindlimb bud	Moderate		3C	Expression in hindlimb regions equivalent to that seen in 3A.

Specimen 4A: embryonic day 11.5 (more

)

Structure	Level	Image
TS19: forelimb bud	Present	4A
TS19: hindlimb bud	Present	4A
TS19: trunk somite	Present	4A

Specimen 4B: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Present		4B

Specimen 4C: embryonic day 11.5; Dmrt2^tm1Rjo/Dmrt2^tm1Rjo, Pax3^Sp/Pax3^Sp (more

)

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Weak		4C	Authors report that the number of expressing cells was considerably lower than that of Pax3+/- Dmrt2+/- embryos or other compound mutants at similar stages.

Specimen 4D: embryonic day 11.5; Dmrt2^tm1Rjo/Dmrt2^tm1Rjo, Pax3^Sp/Pax3^Sp (more

)

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Weak		4D	Authors report that the number of expressing cells was considerably lower than that of Pax3+/- Dmrt2+/- embryos or other compound mutants at similar stages.

Specimen 4E: embryonic day 11.5; Dmrt2^tm1Rjo/Dmrt2^tm1Rjo, Pax3^Sp/Pax3^Sp (more

)

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Weak		4E	Authors report that the number of expressing cells was considerably lower than that of Pax3+/- Dmrt2+/- embryos or other compound mutants at similar stages.

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