Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:113766
Zhang M, et al., Foxj1 regulates asymmetric gene expression during left-right axis patterning in mice. Biochem Biophys Res Commun. 2004 Nov 26;324(4):1413-20
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3841414
|
|
Gene symbol:
|
Pitx2
|
|
Gene name:
|
paired-like homeodomain transcription factor 2
|
|
Probe:
|
Pitx2 probe10
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
5A:
embryonic day 8.0; Foxj1tm1Bph/Foxj1 +
(more  )
Note: This is an early somite stage embryo.
Specimen
5A:
(close  )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * Black Swiss
|
|
Mutant Allele(s):
|
Foxj1tm1Bph/Foxj1+
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
This is an early somite stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
5A
|
In 14 of 14 embryos examined there was expression in the left lateral plate mesoderm but not the right.
|
| TS12: head mesenchyme |
Present |
|
5A
|
In 14 of 14 embryos examined there was bilateral expression in the head mesenchyme.
|
Specimen
5B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * Black Swiss
|
|
Mutant Allele(s):
|
Foxj1tm1Bph/Foxj1tm1Bph
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
This is an early somite stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
5B
|
In 1 of 9 embryos examined there was expression in the right lateral plate mesoderm but not the left.
|
| TS12: head mesenchyme |
Present |
|
5B
|
In 9 of 9 embryos examined there was bilateral expression in the head mesenchyme.
|
Specimen
5C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * Black Swiss
|
|
Mutant Allele(s):
|
Foxj1tm1Bph/Foxj1tm1Bph
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
This is an early somite stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
|
5C
|
In 4 of 9 embryos examined there was expression in both the left and right lateral plate mesoderm.
|
| TS12: head mesenchyme |
Present |
|
5C
|
In 9 of 9 embryos examined there was bilateral expression in the head mesenchyme.
|
Specimen
Not shown:
embryonic day 8.0; Foxj1tm1Bph/ Foxj1tm1Bph
(more )
Note: This is an early somite stage embryo.
Specimen
Not shown:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * Black Swiss
|
|
Mutant Allele(s):
|
Foxj1tm1Bph/Foxj1tm1Bph
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
This is an early somite stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
|
In 1 of 9 embryos examined there was expression in the left lateral plate mesoderm but not the right.
|
Specimen
Not shown:
embryonic day 8.0; Foxj1tm1Bph/ Foxj1tm1Bph
(more )
Note: This is an early somite stage embryo.
Specimen
Not shown:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * Black Swiss
|
|
Mutant Allele(s):
|
Foxj1tm1Bph/Foxj1tm1Bph
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
This is an early somite stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Absent |
|
|
In 3 of 9 embryos examined there was no expression in either the left or right lateral plate mesoderm.
|
Specimen
Not shown:
embryonic day 8.0
(more )
Note: This is an early somite stage embryo.
Specimen
Not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
This is an early somite stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
|
In 8 of 8 embryos examined there was expression in the left lateral plate mesoderm but not the right.
|
| TS12: head mesenchyme |
Present |
|
|
In 8 of 8 embryos examined there was bilateral expression in the head mesenchyme.
|
|
Analysis Tools