Probe:	Nek4 probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Authors state that two probes were used for in situ hybridization and both showed essentially identical patterns. The probe used for this assay was not detailed; for details about the other possible probe see MGI:3841209.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present	Regionally restricted	5A , 5B	Expression was detected in most of the tubules at a low level. Some of the tubules express much higher levels.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Strong	Regionally restricted	5C , 5D	Expression was high in most of the seminiferous tubules.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Strong	Widespread	5F , 5E	High levels of expression are seen in all of the tubules.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatocyte		Present	Regionally restricted	5H , 5G	Expression was present in pachytene spermatocytes.
TS28: spermatid		Present	Regionally restricted	5H , 5G	Expression was detected in round spermatids but not in inner elongated spermatids.
TS28: Leydig cell	Leydig cell	Absent		5G , 5H

Structure	Level	Pattern	Image	Note
TS28: spermatozoon	Absent			Expression was not found in spermatozoa stored in the epididymis.