Nek4 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:56269 Chen A, et al., NIMA-related kinases: isolation and characterization of murine nek3 and nek4 cDNAs, and chromosomal localization of nek1, nek2 and nek3. Gene. 1999 Jun 24;234(1):127-37
Assay type:	Northern blot
MGI Accession ID:	MGI:3841202
Gene symbol:	Nek4
Gene name:	NIMA (never in mitosis gene a)-related expressed kinase 4

Probe:	Nek4 probe1
Probe preparation:	Double stranded DNA
Visualized with:	Phosphorimaging

Results

Image: 4

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	5.3 kb (b)	3.8 kb (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
10.5 dpc	E10.5 (a)	TS17: embryo	Absent	Absent	12 µg; total RNA	C57BL/6J		Not Specified
14.5	E14.5 (a)	TS22: embryo	Absent	Absent	12 µg; total RNA	C57BL/6J		Not Specified
brain	P adult	TS28: brain	Absent	Absent	12 µg; total RNA	C57BL/6J		Not Specified
intestine	P adult	TS28: intestine	Absent	Absent	12 µg; total RNA	C57BL/6J		Not Specified
placenta	E12.5 (a)	TS28: placenta	Absent	Absent	12 µg; total RNA	C57BL/6J		Not Specified
kidney	P adult	TS28: metanephros	Absent	Absent	12 µg; total RNA	C57BL/6J		Not Specified
testis	P adult	TS28: testis	Strong	Strong	12 µg; total RNA	C57BL/6J		Male
ovary	P adult	TS28: ovary	Absent	Absent	12 µg; total RNA	C57BL/6J		Female

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors note that a 1-week exposure revealed faint bands of the same sizes in the E10.5 lane and somewhat fainter bands in all the other tissues examined (data not shown).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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